Lee J H, Kim W H, Cho H, Kwak Y S, Kim S, Paik W K
Department of Biochemistry, Ajou University School of Medicine, Suwon, 442-749, Korea.
Biochem Biophys Res Commun. 1998 Jul 20;248(2):391-4. doi: 10.1006/bbrc.1998.8984.
We previously reported the protective effect of N-carbamoyl-L-glutamate plus L-arginine in rats given a lethal dose (LD99.9) of ammonium acetate (Kim, S. et al., Proc. Natl. Acad. Sci. 69, 3530-3533, 1971; ref.1). The present study was undertaken to find out whether the same compounds could also be effective even after the functional mass of the liver was significantly reduced. Thus, the protective effect of these compounds in 70% partial hepatectomized rats following the injection of sublethal dose of ammonium acetate was assessed. The mixture could significantly decrease blood ammonia level compared with PBS-injected control group. In addition, abnormal behaviors observed in the control rats were significantly improved. The protective effect on the behavioral change seemed to be closely related with their effect on blood ammonia level, showing a strong correlation between the blood ammonia level and the behavioral score. The findings provide a rational basis for the clinical use of N-carbamoyl-L-glutamate plus L-arginine in the prevention and treatment of hyperammonemia encountered in liver diseases.
我们之前报道了N-氨甲酰-L-谷氨酸加L-精氨酸对给予致死剂量（LD99.9）乙酸铵的大鼠的保护作用（Kim, S.等人，《美国国家科学院院刊》69, 3530 - 3533, 1971；参考文献1）。本研究旨在探究即使肝脏功能量显著减少后，相同的化合物是否仍然有效。因此，评估了这些化合物对70%部分肝切除大鼠注射亚致死剂量乙酸铵后的保护作用。与注射PBS的对照组相比，该混合物可显著降低血氨水平。此外，对照组大鼠中观察到的异常行为得到显著改善。对行为变化的保护作用似乎与其对血氨水平的影响密切相关，血氨水平与行为评分之间呈现出强相关性。这些发现为N-氨甲酰-L-谷氨酸加L-精氨酸在预防和治疗肝脏疾病中出现的高氨血症的临床应用提供了合理依据。
