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球形红细菌光捕获复合体I(B875)的模型。

Model for the light-harvesting complex I (B875) of Rhodobacter sphaeroides.

作者信息

Hu X, Schulten K

机构信息

Beckman Institute and Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801 USA.

出版信息

Biophys J. 1998 Aug;75(2):683-94. doi: 10.1016/S0006-3495(98)77558-7.

Abstract

The light-harvesting complex I (LH-I) of Rhodobacter sphaeroides has been modeled computationally as a hexadecamer of alphabeta-heterodimers, based on a close homology of the heterodimer to that of light-harvesting complex II (LH-II) of Rhodospirillum molischianum. The resulting LH-I structure yields an electron density projection map that is in agreement with an 8.5-A resolution electron microscopic projection map for the highly homologous LH-I of Rs. rubrum. A complex of the modeled LH-I with the photosynthetic reaction center of the same species has been obtained by a constrained conformational search. This complex and the available structures of LH-II from Rs. molischianum and Rhodopseudomonas acidophila furnish a complete model of the pigment organization in the photosynthetic membrane of purple bacteria.

摘要

基于球形红杆菌的αβ-异二聚体与莫氏红螺菌光捕获复合物II(LH-II)的异二聚体高度同源,其光捕获复合物I(LH-I)已通过计算建模为αβ-异二聚体的十六聚体。所得的LH-I结构产生了一个电子密度投影图,该图与深红螺菌高度同源的LH-I的8.5埃分辨率电子显微镜投影图一致。通过受限构象搜索获得了建模的LH-I与同一物种光合反应中心的复合物。该复合物以及来自莫氏红螺菌和嗜酸红假单胞菌的LH-II的现有结构为紫色细菌光合膜中的色素组织提供了一个完整模型。

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