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1
CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.来自嗜热菌的甲硫氨酸转运核糖核酸（tRNA-Met-f）中5-甲基-2-硫代尿苷的圆二色光谱
Nucleic Acids Res. 1976 Jul;3(7):1703-13. doi: 10.1093/nar/3.7.1703.
2
CD and NMR studies on the conformational thermostability of 2-thioribothymidine found in the T psi C loop of thermophile tRNA.对嗜热菌转运核糖核酸（tRNA）的TψC环中发现的2-硫代核糖胸苷的构象热稳定性进行的圆二色性（CD）和核磁共振（NMR）研究。
Biochem Biophys Res Commun. 1979 Nov 28;91(2):671-7. doi: 10.1016/0006-291x(79)91574-2.
3
Duplex formation of complementary oligoribonucleotides corresponding to the dihydrouridine loop neck region of several transfer ribonucleic acids.与几种转移核糖核酸的二氢尿嘧啶环颈部区域相对应的互补寡核糖核苷酸的双链体形成。
Can J Biochem. 1977 Apr;55(4):365-8. doi: 10.1139/o77-050.
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The conformation difference between tRNA Met f and formylmethionyl-tRNA Met f from E. coli.来自大肠杆菌的起始甲硫氨酰tRNA（tRNA Met f）与甲酰甲硫氨酰-tRNA（formylmethionyl-tRNA Met f）之间的构象差异。
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6
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Heat-induced stability of tRNA from an extreme thermophile, Thermus thermophilus.
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Circular dichroism study of the interaction of glutamyl-tRNA synthetase with tRNAGlu2.
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Recruitment of divalent metal ions by incorporation of 4-thio-2'-deoxythymidine or 4-thio-2'-deoxyuridine into DNA.通过将4-硫代-2'-脱氧胸苷或4-硫代-2'-脱氧尿苷掺入DNA来招募二价金属离子。
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Synthesis and conformational studies of ribooligonucleotides which contain an alternating C-G sequence and show unusual circular dichroism spectra.含有交替C-G序列并呈现异常圆二色光谱的核糖寡核苷酸的合成与构象研究。
Nucleic Acids Res. 1984 Oct 25;12(20):7793-810. doi: 10.1093/nar/12.20.7793.

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Synthesis and studies on the effect of 2-thiouridine and 4-thiouridine on sugar conformation and RNA duplex stability.2-硫代尿苷和4-硫代尿苷对糖构象及RNA双链稳定性的影响的合成与研究
Nucleic Acids Res. 1997 Mar 15;25(6):1272-80. doi: 10.1093/nar/25.6.1272.
6
Unusual anticodon loop structure found in E.coli lysine tRNA.在大肠杆菌赖氨酸转运核糖核酸中发现的异常反密码子环结构。
Nucleic Acids Res. 1994 Jan 11;22(1):79-87. doi: 10.1093/nar/22.1.79.
7
Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27.嗜热栖热菌HB27的tRNA中2'-O-甲基鸟苷的热诱导生物合成
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1922-6. doi: 10.1073/pnas.77.4.1922.
8
1H NMR studies on the conformational characteristics of 2-thiopyrimidine nucleotides found in transfer RNAs.对转运核糖核酸中发现的2-硫代嘧啶核苷酸构象特征的核磁共振氢谱研究。
Nucleic Acids Res. 1979 Jun 11;6(7):2611-26. doi: 10.1093/nar/6.7.2611.
9
Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.通过质子磁共振监测核糖胸苷在转运RNA热稳定性中的作用。
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10
Occurrence of 1-methyladenosine and absence of ribothymidine in transfer ribonucleic acid of Mycobacterium smegmatis.耻垢分枝杆菌转移核糖核酸中1-甲基腺苷的存在及核糖胸腺嘧啶的缺失
J Bacteriol. 1979 Mar;137(3):1084-7. doi: 10.1128/jb.137.3.1084-1087.1979.

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A specific chemical chain scission of tRNA at 7-methylguanosine.转运核糖核酸（tRNA）在7-甲基鸟苷处的特定化学链断裂。
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Magnesium-induced conformational change in transfer ribonucleic acid as measured by circular dichroism.通过圆二色性测量镁诱导的转移核糖核酸构象变化。
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Selective modification of 4-thiouridylate residue in Escherichia coli transfer RNA with cyanogen bromide.用溴化氰对大肠杆菌转移核糖核酸中的4-硫代尿苷酸残基进行选择性修饰。
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Molecular structure of poly-2-thiouridylic acid, a double helix with non-equivalent polynucleotide chains.聚-2-硫代尿苷酸的分子结构，一种具有不等同多核苷酸链的双螺旋结构。
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来自嗜热菌的甲硫氨酸转运核糖核酸（tRNA-Met-f）中5-甲基-2-硫代尿苷的圆二色光谱

CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.

作者信息

Watanabe K, Oshima T, Nishimura S

出版信息

Nucleic Acids Res. 1976 Jul;3(7):1703-13. doi: 10.1093/nar/3.7.1703.

DOI:10.1093/nar/3.7.1703
PMID:967669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343029/
Abstract

5-Methyl-2-thiouridine (S) in tRNA-Met-f from an extreme thermophile is located in the TpsiC region, replacing T, and has a positive CD band centered at 310 nm. Upon heating, the profiles of the change in this band were similar to the UV melting profiles of the change monitored at 260 nm. This strongly suggests a close relation between heat denaturation of the tRNA and the conformation of the S base. Oligonucleotides containing S showed negative CD bands at 320-330 nm, like the monomer S itself, but when the 3'-2/5 fragment containing S formed a complex with the complementary 5'-3/5 fragment, a positive CD band appeared at 310 nm. These results suggest that combination of the TpsiC loop containing S with the hU loop is necessary for the positive band of S at 310 nm. S may serve to strengthen the association of the TpsiC loop with the hU loop in tRNA of the thermophile.

摘要

来自嗜热菌的tRNA-Met-f中的5-甲基-2-硫代尿苷（S）位于TψC区域，取代了T，并且在310nm处有一个正的圆二色带。加热时，该带变化的图谱与在260nm处监测的紫外熔解图谱相似。这强烈表明tRNA的热变性与S碱基的构象之间存在密切关系。含有S的寡核苷酸在320 - 330nm处显示负的圆二色带，就像单体S本身一样，但是当含有S的3'-2/5片段与互补的5'-3/5片段形成复合物时，在310nm处出现正的圆二色带。这些结果表明，含有S的TψC环与hU环结合对于S在310nm处的正带是必要的。S可能有助于加强嗜热菌tRNA中TψC环与hU环的结合。