Rüssmann H, Shams H, Poblete F, Fu Y, Galán J E, Donis R O
Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA.
Science. 1998 Jul 24;281(5376):565-8. doi: 10.1126/science.281.5376.565.
Avirulent strains of Salmonella typhimurium are being considered as antigen delivery vectors. During its intracellular stage in the host, S. typhimurium resides within a membrane-bound compartment and is not an efficient inducer of class I-restricted immune responses. Viral epitopes were successfully delivered to the host-cell cytosol by using the type III protein secretion system of S. typhimurium. This resulted in class I-restricted immune responses that protected vaccinated animals against lethal infection. This approach may allow the efficient use of S. typhimurium as an antigen delivery system to control infections by pathogens that require this type of immune response for protection.
鼠伤寒沙门氏菌的无毒菌株正被视作抗原递送载体。在宿主细胞内阶段，鼠伤寒沙门氏菌存在于膜结合区室中，并非I类限制性免疫反应的有效诱导剂。通过利用鼠伤寒沙门氏菌的III型蛋白分泌系统，病毒表位成功递送至宿主细胞胞质溶胶。这引发了I类限制性免疫反应，保护接种疫苗的动物免受致死性感染。该方法可能使鼠伤寒沙门氏菌作为抗原递送系统得到有效利用，以控制那些需要此类免疫反应来实现保护的病原体所引发的感染。
