Kirik V, Kölle K, Miséra S, Bäumlein H
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Plant Mol Biol. 1998 Jul;37(5):819-27. doi: 10.1023/a:1006011002499.
Two novel MYB genes (ATMYBR1 and ATMYBR2) were isolated from Arabidopsis thaliana. Binding to a conserved MYB recognition sequence is demonstrated for the ATMYBR1 protein. The expression of both genes is affected by the fus3, lec1 and abi3 mutations causing pleiotropic defects during late embryogenesis and seed maturation including the loss of dormancy and desiccation tolerance. The strong increase of the transcript levels of both MYB genes during very late stages of embryogenesis typically found in wild type is missing in the mutants. Furthermore, the expression of both MYB genes is developmentally regulated in vegetative tissues. The highly conserved repeats (R2 and R3) of the DNA binding MYB domain of both proteins represent chimeric structures combining features typical of plant and animal derived proteins. This demonstrates the existence of a distinct subfamily of animal-like MYB factors in plant genomes.
从拟南芥中分离出两个新的MYB基因（ATMYBR1和ATMYBR2）。已证明ATMYBR1蛋白可与保守的MYB识别序列结合。fus3、lec1和abi3突变会影响这两个基因的表达，这些突变在胚胎后期发育和种子成熟过程中导致多效性缺陷，包括休眠丧失和脱水耐受性丧失。在突变体中，通常在野生型胚胎发育的非常晚期阶段出现的这两个MYB基因转录水平的强烈增加消失了。此外，这两个MYB基因在营养组织中的表达受发育调控。这两种蛋白质的DNA结合MYB结构域的高度保守重复序列（R2和R3）代表了嵌合结构，结合了植物和动物来源蛋白质的典型特征。这证明了植物基因组中存在一个独特的类动物MYB因子亚家族。
