Lotan T, Ohto M, Yee K M, West M A, Lo R, Kwong R W, Yamagishi K, Fischer R L, Goldberg R B, Harada J J
Division of Biological Sciences, University of California, Davis 95616, USA.
Cell. 1998 Jun 26;93(7):1195-205. doi: 10.1016/s0092-8674(00)81463-4.
The Arabidopsis LEAFY COTYLEDON1 (LEC1) gene is required for the specification of cotyledon identity and the completion of embryo maturation. We isolated the LEC1 gene and showed that it functions at an early developmental stage to maintain embryonic cell fate. The LEC1 gene encodes a transcription factor homolog, the CCAAT box-binding factor HAP3 subunit. LEC1 RNA accumulates only during seed development in embryo cell types and in endosperm tissue. Ectopic postembryonic expression of the LEC1 gene in vegetative cells induces the expression of embryo-specific genes and initiates formation of embryo-like structures. Our results suggest that LEC1 is an important regulator of embryo development that activates the transcription of genes required for both embryo morphogenesis and cellular differentiation.
拟南芥叶状子叶1（LEC1）基因对于子叶身份的确定和胚胎成熟的完成是必需的。我们分离了LEC1基因，并表明它在早期发育阶段发挥作用以维持胚胎细胞命运。LEC1基因编码一种转录因子同源物，即CCAAT盒结合因子HAP3亚基。LEC1 RNA仅在种子发育期间在胚胎细胞类型和胚乳组织中积累。LEC1基因在营养细胞中的异位胚后表达诱导胚胎特异性基因的表达并启动胚状结构的形成。我们的结果表明，LEC1是胚胎发育的重要调节因子，可激活胚胎形态发生和细胞分化所需基因的转录。
