哺乳动物植入前发育过程中的染色体甲基化模式。
Chromosome methylation patterns during mammalian preimplantation development.
作者信息
Rougier N, Bourc'his D, Gomes D M, Niveleau A, Plachot M, Pàldi A, Viegas-Péquignot E
机构信息
U 383 Institut National de la Santé et de la Recherche Médicale (INSERM), Necker-Enfants Malades, 75743 Paris Cedex 15, France.
出版信息
Genes Dev. 1998 Jul 15;12(14):2108-13. doi: 10.1101/gad.12.14.2108.
Abstract
DNA methylation patterns were evaluated during preimplantation mouse development by analyzing the binding of monoclonal antibody to 5-methylcytosine (5-MeC) on metaphase chromosomes. Specific chromosome patterns were observed in each cell stage. A banding pattern predominated in chromosomes at the one-cell stage. Banding was replaced at the two-cell stage by an asymmetrical labeling of the sister chromatids. Then, the proportion of asymmetrical chromosomes decreased by one-half at each cell division until the blastocyst stage, and chromosomes became progressively symmetrical and weakly labeled. Our results indicate that chromosome demethylation is associated with each DNA replication and suggest that a passive mechanism predominates during early development.
摘要
通过分析单克隆抗体与中期染色体上5-甲基胞嘧啶（5-MeC）的结合情况，对植入前小鼠发育过程中的DNA甲基化模式进行了评估。在每个细胞阶段都观察到了特定的染色体模式。在单细胞阶段，染色体上主要是条带模式。在双细胞阶段，条带模式被姐妹染色单体的不对称标记所取代。然后，在每次细胞分裂时，不对称染色体的比例减半，直到囊胚阶段，染色体变得逐渐对称且标记较弱。我们的结果表明，染色体去甲基化与每次DNA复制相关，并表明在早期发育过程中主要是被动机制。
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