Yu M, Hansson E, Shiell B, Michalski W, Eaton B T, Wang L F
CSIRO Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria.
J Gen Virol. 1998 Jul;79 ( Pt 7):1775-80. doi: 10.1099/0022-1317-79-7-1775.
The nucleoprotein (N) gene of Hendra virus (HeV), an unclassified member of subfamily Paramyxovirinae in the family Paramyxoviridae previously known as equine morbillivirus, was cloned and sequenced. The majority of the deduced amino acid sequence was further confirmed by direct sequencing of peptide fragments of the N protein derived from purified virions. The 3' untranslated sequence of the HeV N gene mRNA was 568 nt and was much longer than that observed in other Paramyxovirinae. The N protein was 532 amino acids in length with a molecular mass of 58.5 kDa. Although the HeV N protein had a slightly higher amino acid sequence identity to those of the genus Morbillivirus than to those of other Paramyxovirinae genera, the level of identity was much lower than that observed within the morbilliviruses. Our results indicated that HeV could not confidently be classified as a member of the genus Morbillivirus, Paramyxovirus or Rubulavirus and suggest that the virus be classified in a new genus within the Paramyxovirinae.
亨德拉病毒（HeV）曾被称为马麻疹病毒，是副粘病毒科副粘病毒亚科的一个未分类成员，其核蛋白（N）基因被克隆并测序。通过对源自纯化病毒粒子的N蛋白肽片段进行直接测序，进一步证实了推导氨基酸序列的大部分。HeV N基因mRNA的3'非翻译序列为568个核苷酸，比其他副粘病毒亚科中观察到的序列长得多。N蛋白长度为532个氨基酸，分子量为58.5 kDa。虽然HeV N蛋白与麻疹病毒属的氨基酸序列同一性略高于与其他副粘病毒亚科属的同一性，但该同一性水平远低于麻疹病毒属内观察到的水平。我们的结果表明，HeV不能可靠地归类为麻疹病毒属、副粘病毒属或腮腺炎病毒属的成员，并建议将该病毒归类为副粘病毒亚科内的一个新属。
