Department of Microbiology, The University of Hong Kong, Hong Kong, China.
Virology. 2010 Aug 15;404(1):106-16. doi: 10.1016/j.virol.2010.03.049.
Among 489 bats of 11 species in China, three novel paramyxoviruses [Tuhokovirus 1, 2 and 3 (ThkPV-1, ThkPV-2 and ThkPV-3)] were discovered in 15 Leschenault's rousettes. Phylogenetically, the three viruses are most closely related to Menangle and Tioman virus. Genome analysis showed that their 3'-leader sequences are unique by possessing GA instead of AG at the 5th and 6th positions. Unlike Menangle and Tioman virus, key amino acids for neuraminidase activity characteristic of rubulavirus attachment proteins are present. The genome of ThkPV-1 represents the largest rubulavirus genome. Unique features between the three viruses include perfect complementary 5'-trailer and 3'-leader sequence and a unique cysteine pair in attachment protein of ThkPV-1, G at +1 position in all predicted mRNA sequences of ThkPV-2, and amino acid substitutions in the conserved N-terminal motif of nucleocapsid of ThkPV-3. Analysis of phosphoprotein gene mRNA products confirmed mRNA editing. Antibodies to the viruses were detected in 48-60% of Leschenault's rousettes.
在中国 11 种蝙蝠的 489 只蝙蝠中，在 15 只 Leschenault 的 Rousettes 中发现了三种新型副粘病毒[Tuhokovirus 1、2 和 3（ThkPV-1、ThkPV-2 和 ThkPV-3）]。从系统进化的角度来看，这三种病毒与 Menangle 和 Tioman 病毒最为密切相关。基因组分析表明，它们的 3' - 前导序列是独特的，第 5 位和第 6 位是 GA，而不是 AG。与 Menangle 和 Tioman 病毒不同，具有神经氨酸酶活性的特征的关键氨基酸附着蛋白存在于 rubulavirus 中。ThkPV-1 的基因组代表了最大的 rubulavirus 基因组。三种病毒之间的独特特征包括完美互补的 5'- 尾和 3'- 前导序列以及 ThkPV-1 附着蛋白中的独特半胱氨酸对，ThkPV-2 的所有预测 mRNA 序列中+1 位的 G，以及 ThkPV-3 的核衣壳保守 N 端基序中的氨基酸取代。磷蛋白基因 mRNA 产物分析证实了 mRNA 编辑。在 48-60%的 Leschenault Rousettes 中检测到了针对这些病毒的抗体。
