Collins A R, Raslová K, Somorovská M, Petrovská H, Ondrusová A, Vohnout B, Fábry R, Dusinská M
Rowett Research Institute, Aberdeen, Scotland, UK.
Free Radic Biol Med. 1998 Aug;25(3):373-7. doi: 10.1016/s0891-5849(98)00053-7.
Levels of DNA damage in groups of 10 patients with insulin-dependent diabetes mellitus and 10 matched controls were compared using the comet assay; DNA strand breaks, oxidized pyrimidines (endonuclease III-sensitive sites) and altered purines (sites sensitive to formamidopyrimidine glycosylase) were measured. Mean values of strand breaks and oxidized pyrimidines were significantly higher in diabetics. Strand breaks correlated with body mass index in the diabetic group. A strong correlation was seen between formamidopyrimidine glycosylase-sensitive sites and serum glucose concentrations. When three patients with normal glucose levels were excluded from the statistical analysis, the mean value of formamidopyrimidine glycosylase-sensitive sites was very significantly elevated compared with normal. DNA damage in lymphocytes is thus a useful marker of oxidative stress, and in particular formamidopyrimidine glycosylase-sensitive sites seem to represent changes specifically related to hyperglycemia.
采用彗星试验比较了10例胰岛素依赖型糖尿病患者和10例配对对照者的DNA损伤水平；检测了DNA链断裂、氧化嘧啶（核酸内切酶III敏感位点）和改变的嘌呤（对甲酰胺嘧啶糖基化酶敏感的位点）。糖尿病患者的链断裂和氧化嘧啶的平均值显著更高。糖尿病组中链断裂与体重指数相关。在对甲酰胺嘧啶糖基化酶敏感位点与血清葡萄糖浓度之间观察到强相关性。当将3例血糖水平正常的患者排除在统计分析之外时，与正常情况相比，对甲酰胺嘧啶糖基化酶敏感位点的平均值非常显著升高。因此，淋巴细胞中的DNA损伤是氧化应激的一个有用标志物，尤其是对甲酰胺嘧啶糖基化酶敏感位点似乎代表了与高血糖症特别相关的变化。
