Jarrell K F, Vydykhan T, Lee P, Agnew M D, Thomas N A
Department of Microbiology and Immunology, Queen's University, Kingston, ON, Canada.
Extremophiles. 1997 Nov;1(4):199-206. doi: 10.1007/s007920050034.
The isolation and characterization of a novel bacteriophage active against the obligately alkaliphilic bacterium Bacillus clarkii is described. The bacteriophage, designated BCJA1. is a member of the Siphoviridae family with a B1 morphology. It possesses an isometric head, which measures 65 nm between opposite apices, and a noncontractile tail of 195 nm length. It had a buoyant density of 1.518 g/ml and an estimated particle mass of 37 x 10(7) daltons. BCJA1 was stable over the pH range of 6-11. A one-step growth experiment conducted at pH 10 demonstrated a latent period of about 40 min and a burst size of approximately 40. The purified bacteriophage appeared to consist of 10 proteins with the major head and tail proteins likely to be of molecular weight 36500 and 28000, respectively. The genome size was estimated to be between 32.1 and 34.8 kb. The percent G + C content of purified bacteriophage DNA was 45.6. The wildtype bacteriophage is temperate but a clear plaque mutant was isolated.
本文描述了一种对专性嗜碱菌克拉克芽孢杆菌具有活性的新型噬菌体的分离与特性。该噬菌体命名为BCJA1，属于具有B1形态的长尾噬菌体科。它有一个等轴状头部，相对顶点间距离为65纳米，还有一条195纳米长的非收缩性尾部。其浮力密度为1.518克/毫升，估计颗粒质量为37×10⁷道尔顿。BCJA1在pH值6至11的范围内稳定。在pH值10下进行的一步生长实验表明，潜伏期约为40分钟，裂解量约为40。纯化后的噬菌体似乎由10种蛋白质组成，主要头部和尾部蛋白质的分子量可能分别为36500和28000。基因组大小估计在32.1至34.8千碱基之间。纯化噬菌体DNA的G + C含量百分比为45.6。野生型噬菌体是温和型的，但分离出了一个清亮噬菌斑突变体。
