Hermo L, Smith C E
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada.
Histochem Cell Biol. 1998 May-Jun;109(5-6):431-47. doi: 10.1007/s004180050246.
The Golgi apparatus of epididymal principal cells shares many structural features with other cell types. Saccular regions are arranged in a cis-Golgi network, eight flattened saccules, and several trans-Golgi networks (TGNs). Dilated tubules form intersaccular connecting regions which joint together saccules at the same or different levels between adjacent stacks. Wells exist as large perforations in register with the four cis-most saccules and serve as areas of vesicular interactions. TGNs are variable and can appear to peel off the stack or to be detached from it in the form of an anastomotic tubular network with pale dilated areas corresponding to prosecretory granules connected by short narrow bridges. Elongated or discoid dilated cisternae of endoplasmic reticulum (ER) (sparsely granulated) lie over the cis face of the stack, from which they are separated by an intermediate compartment filled with vesicles and tubules. The ER is also closely juxtaposed to the TGNs and the eighth saccule but interconnections are never seen between them. Vesicles of the COP variety reside at all levels of the stack and appear to bud off the cis-located ER and the edges of the saccules, while clathrin-coated vesicles appear mainly on the trans face of the stack and next to lysosomes. In the supranuclear cytoplasm, clusters of vesicles and tubules, at times budding off enveloping ER, appear to radiate toward the Golgi stacks where they fuse with cis Golgi elements. Taken together, these observations suggest dynamic functions and interactions for the various Golgi elements, associated vesicles, ER, and vesicular tubular clusters.
附睾主细胞的高尔基体与其他细胞类型具有许多结构特征。囊状区域排列成顺面高尔基体网络、八个扁平囊泡和几个反面高尔基体网络（TGNs）。扩张的小管形成囊泡间连接区域，在相邻堆叠的相同或不同水平处将囊泡连接在一起。小孔作为与最靠近顺面的四个囊泡对齐的大穿孔存在，并作为囊泡相互作用的区域。TGNs是可变的，可能看起来从堆叠上剥离或以吻合管状网络的形式从其分离，具有对应于通过短窄桥连接的分泌前颗粒的淡染扩张区域。内质网（ER）的细长或盘状扩张的潴泡（颗粒稀疏）位于堆叠的顺面上方，它们与堆叠之间由充满囊泡和小管的中间隔室隔开。ER也与TGNs和第八个囊泡紧密并列，但从未在它们之间看到相互连接。COP种类的囊泡存在于堆叠的所有水平，似乎从位于顺面的ER和囊泡边缘芽生，而网格蛋白包被的囊泡主要出现在堆叠的反面和溶酶体旁边。在核上区细胞质中，有时从包绕的ER芽生的囊泡和小管簇似乎向高尔基体堆叠辐射，在那里它们与顺面高尔基体元件融合。综上所述，这些观察结果表明各种高尔基体元件、相关囊泡、ER和囊泡管状簇具有动态功能和相互作用。
