Marmillot P, Satchithanandam S, Liu Q H, Lakshman M R
Department of Medicine, George Washington University, Washington, DC 20037, USA.
Nutr Cancer. 1998;31(1):18-23. doi: 10.1080/01635589809514673.
We have determined the influence of dietary taurocholate and beta-carotene on the absorption and biotransformation of newly administered beta-14C]carotene. Male ferrets were fed the control or beta-carotene diet (0.05% beta carotene wt/wt) with and without taurocholate (1% wt/wt) for four weeks, and then the absorption and biotransformation of newly administered beta-[14C]carotene was measured after eight hours in intact or thoracic lymph duct-cannulated animals. Percent recover of beta-[14C]carotene in the liver was increased 3.6-fold (p < 0.05) in the taurocholate-fed ferrets regardless of whether they were fed the control or beta-carotene diet. Percent recovery of labeled vitamin A in the liver was also increased by the same magnitude (p < 0.05). These results were confirmed in thoracic lymph duct-cannulated ferrets. The recoveries of beta-carotene label in the lymph were comparable to the corresponding values in livers of intact animals. The recovery of beta-carotene label in the liver was 50% (p M 0.05) higher in beta-carotene-fed than in control animals. Taurocholate stimulates intestinal absorption of newly administered beta [14C]carotene and its metabolic conversion to 14C-labeled vitamin A (retinol + retinyl ester) 3.6-fold. Beta-Carotene absorption is as efficient in thoracic lymph duct-cannulated ferrets as in intact animals. Prior beta-carotene feeding also stimulates the absorption of newly administered beta-carotene by 50%.
我们已经确定了膳食牛磺胆酸盐和β-胡萝卜素对新给予的β-[¹⁴C]胡萝卜素吸收和生物转化的影响。雄性雪貂分别喂食对照饮食或β-胡萝卜素饮食（0.05%β-胡萝卜素重量/重量），添加或不添加牛磺胆酸盐（1%重量/重量），持续四周，然后在完整动物或胸导管插管动物中，八小时后测量新给予的β-[¹⁴C]胡萝卜素的吸收和生物转化。无论喂食对照饮食还是β-胡萝卜素饮食，喂食牛磺胆酸盐的雪貂肝脏中β-[¹⁴C]胡萝卜素的回收率提高了3.6倍（p<0.05）。肝脏中标记维生素A的回收率也提高了相同幅度（p<0.05）。这些结果在胸导管插管的雪貂中得到了证实。淋巴中β-胡萝卜素标记物的回收率与完整动物肝脏中的相应值相当。喂食β-胡萝卜素的动物肝脏中β-胡萝卜素标记物的回收率比对照动物高50%（p<0.05）。牛磺胆酸盐刺激新给予的β-[¹⁴C]胡萝卜素的肠道吸收及其向¹⁴C标记的维生素A（视黄醇+视黄酯）的代谢转化达3.6倍。在胸导管插管的雪貂中，β-胡萝卜素的吸收与完整动物一样高效。预先喂食β-胡萝卜素也会使新给予的β-胡萝卜素的吸收提高50%。
