Burkin D J, Broad T E, Lambeth M R, Burkin H R, Jones C
AgResearch, Department of Biochemistry, Dunedin, New Zealand.
Anim Genet. 1998 Feb;29(1):48-54. doi: 10.1046/j.1365-2052.1998.00253.x.
The generation and characterization of new sheep-hamster cell hybrids is reported from the fusion of sheep white blood cells with six different hamster auxotrophs. Selection from these and previously generated cell hybrids has led to the production of a panel of 30 hybrids covering the complete sheep genome of 28 chromosomes. Over half of the cell hybrids in this panel contain single sheep chromosomes. By complementation, the following new assignments have been made using the panel: phosphoribosyl N-formylglycinamide amidotransferase (PRFGA) to sheep chromosome (chr) 11; adenylosuccinate synthetase (ADSS) to sheep chr 12; adenylosuccinate lyase (ADSL) to sheep chr 3q; 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGCS) to sheep chr 16; dihydrofolate reductase (DHFR) to sheep chr 5; and adenine phosphoribosyltransferase (APRT) to sheep chr 14. The gene phosphoribosylaminoinidazole-carboxamide formyltransferase/Inosinicase (PRACFT) has now been regionally assigned to chr 2q. By isozyme analysis, phosphogluconate dehydrogenase (PGD) was assigned to sheep chr 12, anchoring the sheep syntenic group U1 to this chromosome, and mannose phosphate isomerase (MPI) was assigned to sheep chr 18. Furthermore, the chromosomal assignment of 110 microsatellites was confirmed using this cell panel.
本文报道了绵羊白细胞与六种不同仓鼠营养缺陷型细胞融合产生并鉴定新的绵羊 - 仓鼠细胞杂种的过程。从这些以及之前产生的细胞杂种中进行筛选，已培育出一组涵盖28条染色体完整绵羊基因组的30个杂种细胞。该组中超过一半的细胞杂种含有单条绵羊染色体。通过互补作用，利用该细胞组进行了以下新的基因定位：磷酸核糖N - 甲酰甘氨酰胺转酰胺酶（PRFGA）定位于绵羊染色体（chr）11；腺苷酸琥珀酸合成酶（ADSS）定位于绵羊chr 12；腺苷酸琥珀酸裂解酶（ADSL）定位于绵羊chr 3q；3 - 羟基 - 3 - 甲基戊二酰辅酶A合酶（HMGCS）定位于绵羊chr 16；二氢叶酸还原酶（DHFR）定位于绵羊chr 5；腺嘌呤磷酸核糖转移酶（APRT）定位于绵羊chr 14。磷酸核糖氨基咪唑 - 羧酰胺甲酰转移酶/肌苷酸酶（PRACFT）基因现已被定位到chr 2q区域。通过同工酶分析，磷酸葡萄糖酸脱氢酶（PGD）定位于绵羊chr 12，从而将绵羊同线群U1锚定到该染色体上，磷酸甘露糖异构酶（MPI）定位于绵羊chr 18。此外，利用该细胞组证实了110个微卫星的染色体定位。
