Monteiro V G, Soares C P, de Souza W
Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, RJ, Brazil.
FEMS Microbiol Lett. 1998 Jul 15;164(2):323-7. doi: 10.1111/j.1574-6968.1998.tb13105.x.
Tachyzoites of Toxoplasma gondii are able to infect several cell types tested (wild-type chinese hamster ovary (CHO) cells and glycosylation mutants, Vero and LLCMK2 cells). However, the extent of infection varied. Mutant cells which present few or no surface-exposed sialic acid residues were infected to a lower extent. Similar results were obtained if sialic acid residues were removed by previous neuraminidase treatment. Addition of sialic acid residues to surface-exposed glycoconjugates using fetuin as a sialic acid donor and the trans-sialidase of Trypanosoma cruzi rendered the cells more easily infected by Toxoplasma gondii. These observations indicate that surface-exposed carbohydrate residues of the host cell are involved on the process of Toxoplasma gondii-host cell recognition.
刚地弓形虫速殖子能够感染所测试的几种细胞类型（野生型中国仓鼠卵巢（CHO）细胞和糖基化突变体、非洲绿猴肾细胞（Vero）和恒河猴肾细胞（LLCMK2））。然而，感染程度有所不同。表面几乎没有或没有唾液酸残基的突变细胞被感染的程度较低。如果先用神经氨酸酶处理去除唾液酸残基，也会得到类似的结果。使用胎球蛋白作为唾液酸供体，并利用克氏锥虫的转唾液酸酶将唾液酸残基添加到表面暴露的糖缀合物上，使细胞更容易被刚地弓形虫感染。这些观察结果表明，宿主细胞表面暴露的碳水化合物残基参与了刚地弓形虫与宿主细胞的识别过程。
