假定的TCP-1伴侣蛋白是参与由……介导的唾液酸依赖性宿主细胞入侵的重要因子。 （注：原文中“by”后面内容缺失）
The Putative TCP-1 Chaperonin Is an Important Player Involved in Sialic Acid-Dependent Host Cell Invasion by .
作者信息
Yang Na, Xing Mengen, Ding Yingying, Wang Dawei, Guo Xiaogai, Sang Xiaoyu, Li Jiaqi, Li Chenghuan, Wang Yanhu, Feng Ying, Chen Ran, Wang Xinyi, Jiang Ning, Chen Qijun
机构信息
Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, Shenyang, China.
出版信息
Front Microbiol. 2020 Feb 21;11:258. doi: 10.3389/fmicb.2020.00258. eCollection 2020.
Host cell invasion by is crucial for the survival and proliferation of parasite. The process of tachyzoite invasion requires interaction between parasite proteins and receptors on the surface of host cells. Sialic acid is one of the important receptors for host cell invasion by . However, the parasite-derived proteins interacting with sialic acid have not been well characterized. In this study, a novel protein named putative TCP-1 chaperonin (TGME49_318410) in (TgTCP-1) was targeted and characterized. TgTCP-1 protein colocalized with MIC3 protein, which could be secreted from tachyzoites, and this protein showed a specific binding activity to sialic acid, and DC and Vero cells . The binding of TgTCP-1 protein to DC and Vero cells were inhibited by either pre-incubation with free sialic acid or neuraminidase treatment of the cells. Moreover, a significant reduction of invasion in Vero cells was observed after pre-incubation of the cells with recombinant TgTCP-1 protein. These results illustrated that TgTCP-1 is an important molecule involved in sialic acid-dependent host cell invasion by .
速殖子对宿主细胞的侵袭对于寄生虫的存活和增殖至关重要。速殖子的侵袭过程需要寄生虫蛋白与宿主细胞表面受体之间的相互作用。唾液酸是速殖子侵袭宿主细胞的重要受体之一。然而，与唾液酸相互作用的寄生虫衍生蛋白尚未得到很好的表征。在本研究中，对一种名为推定TCP-1伴侣蛋白（TgTCP-1）的新型蛋白进行了靶向和表征。TgTCP-1蛋白与可从速殖子分泌的MIC3蛋白共定位，并且该蛋白对唾液酸以及DC细胞和Vero细胞表现出特异性结合活性。用游离唾液酸预孵育或对细胞进行神经氨酸酶处理均可抑制TgTCP-1蛋白与DC细胞和Vero细胞的结合。此外，用重组TgTCP-1蛋白预孵育细胞后，观察到Vero细胞中速殖子的侵袭显著减少。这些结果表明，TgTCP-1是参与速殖子依赖唾液酸的宿主细胞侵袭的重要分子。
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