木糖磷酸酶（xylP）在植物乳杆菌中的功能性表达，该酶编码戊糖乳杆菌的异麦芽糖转运蛋白。
Functional expression in Lactobacillus plantarum of xylP encoding the isoprimeverose transporter of Lactobacillus pentosus.
作者信息
Chaillou S, Postma P W, Pouwels P H
机构信息
EC Slater Institute, BioCentrum, University of Amsterdam, 1018 TV Amsterdam, The Netherlands.
出版信息
J Bacteriol. 1998 Aug;180(15):4011-4. doi: 10.1128/JB.180.15.4011-4014.1998.
Abstract
The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter. Functional expression of the XylP transporter was shown by uptake of isoprimeverose in L. plantarum 80 cells, and this transport was driven by the proton motive force generated by malolactic fermentation. XylP was unable to catalyze transport of D-xylose.
摘要
戊糖乳杆菌的木糖转运蛋白基因（xylP）是木糖转运蛋白操纵子（xylPQR）的首个基因，最近发现它参与异麦芽糖代谢。通过在植物乳杆菌80（一株缺乏活性异麦芽糖和D-木糖转运活性的菌株）的多拷贝质粒上表达xylP，结果表明xylP编码一种转运蛋白。植物乳杆菌80细胞中异麦芽糖的摄取显示了木糖转运蛋白XylP的功能表达，并且这种转运是由苹果酸乳酸发酵产生的质子动力驱动的。XylP无法催化D-木糖的转运。
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