Schönfelder U, Hofer A, Paul M, Funk R H
Institut für Anatomie der TU Dresden, Dresden, D-01307, Germany.
Microvasc Res. 1998 Jul;56(1):22-9. doi: 10.1006/mvre.1998.2086.
We observed the retinal capillary pericytes of the rat in situ. Whole retinae were mounted, immediately post vivo, in a special tissue chamber for electronic light microscopy at high magnifications. Under electronic light microscopy the pericytes could be clearly distinguished from the endothelial cells. In addition, the contractile apparatus of the pericytes was demonstrated by immunohistochemistry with alpha-smooth muscle actin. Administration of angiotensin II as well as endothelin into the observation chamber caused a significant decrease of the mean capillary diameter (13 and 16% reduction, respectively) within 90 s. Carbachol, bradykinin, and histamine significantly increased the capillary diameter within 90 s (13, 20, and 18% increase, respectively). This study demonstrates that our method allows the analysis of vasoactive effects on the retinal capillary in situ. We observed that this type of capillary can actively change its diameter.
我们对大鼠视网膜毛细血管周细胞进行了原位观察。在大鼠死后立即将整个视网膜置于一个特殊的组织腔室中，用于高倍电子光学显微镜观察。在电子光学显微镜下，周细胞可与内皮细胞清晰区分。此外，通过α-平滑肌肌动蛋白免疫组织化学法证实了周细胞的收缩装置。向观察腔室中注入血管紧张素II和内皮素后，在90秒内平均毛细血管直径显著减小（分别降低13%和16%）。卡巴胆碱、缓激肽和组胺在90秒内可显著增加毛细血管直径（分别增加13%、20%和18%）。本研究表明，我们的方法可用于原位分析对视网膜毛细血管的血管活性作用。我们观察到这种类型的毛细血管能够主动改变其直径。
