Okihashi M, Obana H, Hori S
Osaka Prefectural Institute of Public Health, Japan.
Analyst. 1998 Apr;123(4):711-4. doi: 10.1039/a707717c.
Residual N-methylcarbamate pesticides in food were determined by accelerated solvent extraction (ASE) and HPLC with post-column fluorescence. Pesticides were extracted with acetonitrile at 100 degrees C under 2000 psi pressure in less than 20 min. Extracts were cleaned-up with a carboxylic acid mini-column eluted with 10% or 30% acetone in hexane. Eight foods were spiked with 17 pesticides at 0.2 ppm. The average recoveries of these pesticides were 70-100% and the relative standard deviations were < 10%. These results suggested that ASE can be used to extract residues of N-methylcarbamate pesticides in foods.
采用加速溶剂萃取（ASE）和柱后荧光检测的高效液相色谱法（HPLC）测定食品中的残留氨基甲酸甲酯类农药。在2000 psi压力下，于100℃用乙腈萃取农药，萃取时间不到20分钟。萃取液用羧酸微型柱净化，用10%或30%的丙酮己烷溶液洗脱。向8种食品中添加17种浓度为0.2 ppm的农药。这些农药的平均回收率为70-100%，相对标准偏差<10%。这些结果表明，ASE可用于萃取食品中的氨基甲酸甲酯类农药残留。
