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用卢莫加里昂染色后,通过共聚焦激光扫描显微镜对生物组织中的痕量铝进行灵敏检测。

Sensitive detection of trace aluminium in biological tissues by confocal laser scanning microscopy after staining with lumogallion.

作者信息

Uchiumi A, Takatsu A, Teraki Y

机构信息

National Institute of Material and Chemical Research, Ibaraki, Japan.

出版信息

Analyst. 1998 Apr;123(4):759-62. doi: 10.1039/a704876i.

DOI:10.1039/a704876i
PMID:9684408
Abstract

This paper describes a method for the sensitive detection of aluminium in biological tissues by real time confocal laser scanning microscopy after staining with lumogallion. The method enabled detection of aluminium > or = 9 micrograms g-1 in bone and is more sensitive than the conventional histochemical methods with aluminium and solochrome azurine, etc. Lumogallion reacts specifically with aluminium to form a fluorescent complex so that the proposed method is useful for detection and identification of aluminium in tissues.

摘要

本文介绍了一种通过用荧光镓试剂染色后利用实时共聚焦激光扫描显微镜灵敏检测生物组织中铝的方法。该方法能够检测骨中铝含量≥9微克/克,且比用铝和铬天蓝等的传统组织化学方法更灵敏。荧光镓试剂与铝特异性反应形成荧光复合物,因此该方法有助于检测和鉴定组织中的铝。

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