Gaucher S P, Leary J A
Department of Chemistry, University of California, Berkeley 94720, USA.
Anal Chem. 1998 Jul 15;70(14):3009-14. doi: 10.1021/ac980023k.
Diastereomeric diethylenetriamine N-glycoside zinc(II) complexes are investigated using electrospray ionization followed by tandem mass spectrometry in a quadrupole ion trap. Dissociation ions specific to stereochemical differences at C2 and C4 in hexose complexes are observed in the MS2 and MS3 spectra, thus allowing unambiguous differentiation of glucose, galactose, mannose, and talose. Labeling studies incorporating 2H and 13C are used to probe the mechanisms of dissociation involved with these diastereomers, and MS2 studies on deoxyglucose complexes are implemented to support proposed sites of deprotonation within the complexes.
使用电喷雾电离，随后在四极杆离子阱中进行串联质谱分析，对非对映异构的二亚乙基三胺N - 糖苷锌（II）配合物进行了研究。在MS2和MS3光谱中观察到己糖配合物中C2和C4处立体化学差异所特有的解离离子，从而能够明确区分葡萄糖、半乳糖、甘露糖和塔罗糖。采用含2H和13C的标记研究来探究这些非对映异构体的解离机制，并对脱氧葡萄糖配合物进行MS2研究，以支持配合物中质子脱除的推测位点。
