Akashi K, Grandjean O, Small I
Station de Génétique et Amélioration des Plantes, INRA, Versailles, France.
FEBS Lett. 1998 Jul 10;431(1):39-44. doi: 10.1016/s0014-5793(98)00717-0.
A cDNA clone encoding a histidyl-tRNA synthetase (HisRS) was characterized from Arabidopsis thaliana. The deduced amino acid sequence (AtHRS1) is surprisingly more similar to HisRSs from archaebacteria than those from eukaryotes and prokaryotes. AtHRS1 has an N-terminal extension with features characteristic of mitochondrial and chloroplast transit peptides. Transient expression assays in tobacco protoplasts clearly demonstrated efficient targeting of a fusion peptide consisting of the first 71 amino acids of AtHRS1 joined to jellyfish green fluorescent protein (GFP) to both mitochondria and chloroplasts. These observations suggest that the AtHisRS1 cDNA encodes both mitochondrial and chloroplast histidyl-tRNA synthetases.
从拟南芥中鉴定出一个编码组氨酰 - tRNA合成酶（HisRS）的cDNA克隆。推导的氨基酸序列（AtHRS1）与古细菌的HisRSs惊人地更相似，而不是与真核生物和原核生物的HisRSs相似。AtHRS1具有N端延伸，具有线粒体和叶绿体转运肽的特征。烟草原生质体中的瞬时表达分析清楚地表明，由AtHRS1的前71个氨基酸与水母绿色荧光蛋白（GFP）连接组成的融合肽有效地靶向线粒体和叶绿体。这些观察结果表明，AtHisRS1 cDNA编码线粒体和叶绿体组氨酰 - tRNA合成酶。
