Yang Y P, Womack J E
Department of Veterinary Pathobiology and Center for Animal Genetics, Institute of Biosciences and Technology, Texas A&M University, College Station, Texas 77843-4467 USA.
Genome Res. 1998 Jul;8(7):731-6. doi: 10.1101/gr.8.7.731.
Comparative gene mapping in mammals typically involves identification of segments of conserved synteny in diverse genomes. The development of maps that permit comparison of gene order within conserved synteny has not advanced beyond the mouse map that takes advantage of linkage analysis in interspecific backcrosses. Radiation hybrid (RH) mapping provides a powerful tool for determining order of genes in genomes for which gene-based linkage mapping is impractical. Comparative RH mapping of 24 orthologous genes in this study revealed internal structural rearrangements between human chromosome 17 (HSA17) and bovine chromosome 19 (BTA19), two chromosomes known previously to be conserved completely and exclusively at level of synteny. Only six of the 24 genes had been previously ordered on the human G3 RH map. The use of the G3 panel to map the other 18, however, produced parallel RH maps for comparison of gene order at a resolution of <5 Mb on the bovine linkage map and from 1 to 3 Mb in the human physical map.
哺乳动物中的比较基因图谱绘制通常涉及在不同基因组中识别保守同线性片段。能够比较保守同线性区域内基因顺序的图谱开发，目前尚未超越利用种间回交中的连锁分析构建的小鼠图谱。辐射杂种（RH）图谱绘制为确定那些基于基因的连锁图谱绘制不实用的基因组中的基因顺序提供了强大工具。本研究中对24个直系同源基因的比较RH图谱绘制揭示了人类17号染色体（HSA17）和牛19号染色体（BTA19）之间的内部结构重排，这两条染色体此前已知在同线性水平上完全且唯一地保守。24个基因中只有6个此前已在人类G3 RH图谱上排序。然而，使用G3板对其他18个基因进行图谱绘制，产生了平行的RH图谱，以便在牛连锁图谱上以小于5 Mb的分辨率以及在人类物理图谱上以1至3 Mb的分辨率比较基因顺序。
