Gordenin D A, Resnick M A
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, 101 Alexander Dr., P.O. Box 12233, Research Triangle Park, NC 27709, USA.
Mutat Res. 1998 May 25;400(1-2):45-58. doi: 10.1016/s0027-5107(98)00047-5.
The genomes of all organisms contain an abundance of DNA repeats which are at-risk for causing genetic change. We have used the yeast Saccharomyces cerevisiae to investigate various repeat categories in order to understand their potential for causing genomic instability and the role of DNA metabolism factors. Several types of repeats can increase enormously the likelihood of genetic changes such as mutation or recombination when present either in wild type or mutants defective in replication or repair. Specifically, we have investigated inverted repeats, homonucleotide runs, and short distant repeats and the consequences of various DNA metabolism mutants. Because the at-risk motifs (ARMs) that we characterized are sensitive indicators, we have found that they are useful tools to reveal new genetic factors affecting genome stability as well as to distinguish subtle differences between alleles.
所有生物体的基因组都含有大量有引发基因变化风险的DNA重复序列。我们利用酿酒酵母来研究各类重复序列，以了解它们引发基因组不稳定的可能性以及DNA代谢因子的作用。当几种类型的重复序列存在于野生型或复制或修复有缺陷的突变体中时，它们会极大地增加诸如突变或重组等基因变化的可能性。具体而言，我们研究了反向重复序列、同核苷酸序列和短距离重复序列以及各种DNA代谢突变体的后果。由于我们所鉴定的风险基序（ARM）是敏感指标，我们发现它们是揭示影响基因组稳定性的新遗传因子以及区分等位基因间细微差异的有用工具。
