Liu H G, Hong G X, Wang F B, Chen F
Department of Hand Surgery, Union Hospital of Tongji Medical University, Wuhan 430022, China.
Brain Res. 1998 Aug 3;800(2):216-26. doi: 10.1016/s0006-8993(98)00499-5.
The effects of limb bud-derived motoneurotrophins (LBMNTs) as seen in the motoneurons in the anterior spinal cord and sciatic nerve regeneration of adult rats, were evaluated in the present study. A nerve regeneration chamber with a nerve gap of 9 mm was created by suturing the proximal and distal ends of a random sciatic nerve into a silicone tube after removal of a 5 mm piece of nerve in the distal end, The chamber of the experimental group was filled with 34.34 microg LBMNTs and PBS (0.01 mol/ml, pH 7.0),and the control group with PBS only. At 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks post surgery, the content of acetylcholine esterase (AchE) and acid phosphatase (ACP) of the anterior spinal cord (injured side) was quantified, and the corresponding motoneuron's ultrastructure and the existant ratio were also examined. Meanwhile, the regenerated nerve from within the silicone tube was examined at 2, 4 and 6 weeks post surgery for histological studies at both the light microscopic and ultrastructural levels. The experimental group showed a smaller decrease of AchE and an increase of ACP, a larger existant ratio of motoneurons, better ultrastructure and a more mature regenerated nerve based on a larger diameter of the regenerated nerve trunk, a greater number of axons and thicker myelin sheaths than the control group. So it was concluded that LBMNTs had a high activity of protecting motoneurons in the anterior spinal cord after nerve injury and promoting nerve regeneration, and it may be a new source of neurotrophic factors (NTFs).
在本研究中，评估了肢体芽衍生的运动神经营养因子（LBMNTs）对成年大鼠脊髓前角运动神经元及坐骨神经再生的影响。在切除坐骨神经远端5mm的一段神经后，将坐骨神经的近端和远端随机缝合到硅胶管中，形成一个神经间隙为9mm的神经再生室。实验组的腔室中填充34.34μg的LBMNTs和PBS（0.01mol/ml，pH7.0），对照组仅填充PBS。在术后1天、4天、1周、2周、4周和6周时，对脊髓前角（损伤侧）的乙酰胆碱酯酶（AchE）和酸性磷酸酶（ACP）含量进行定量，并检查相应运动神经元的超微结构和存活比例。同时，在术后2周、4周和6周时，对硅胶管内再生神经进行光镜和超微结构水平的组织学研究。与对照组相比，实验组AchE的下降幅度较小，ACP升高，运动神经元的存活比例更高，超微结构更好，再生神经更成熟，表现为再生神经干直径更大、轴突数量更多、髓鞘更厚。因此得出结论，LBMNTs在神经损伤后对脊髓前角运动神经元具有较高的保护活性，并能促进神经再生，可能是一种新的神经营养因子来源。
