Pang Q J, Luo Y X, Wu Y G, Li Z F, Fang H, Yuan T Z, Zhang W R, Chen Q W
Department of Orthopedics, Tongji Hospital, Tongi Medical University, Wuhan.
J Tongji Med Univ. 1993;13(1):34-9. doi: 10.1007/BF02886591.
The effect of exogenous nerve growth factor (NGF) examined on the neural repair of adult rabbit sciatic nerve was evaluated in the present study. A nerve regeneration chamber was created by suturing the proximal and distal ends of a transected sciatic nerve into a silicone chamber. A gap of 6 mm in chamber was left after removal of a 3 mm piece of nerve in the distal ends and insertion of the proximal and distal stumps into the chamber. Animals were operated on bilaterally, one side of the chamber was filled with a 1 mg/ml NGF/normal saline (NS) (experimental) and the contralateral side with NS (control). The regenerated nerves from within the silicone chamber were dissected and fixed 1 to 5 weeks following surgery for histological studies at both the light microscopic and ultrastructural levels. The NGF chamber showed a more mature regenerated nerve based on a larger diameter of the regenerated nerve trunk, a great number of axons, and thicker myelin sheaths.
本研究评估了外源性神经生长因子（NGF）对成年兔坐骨神经神经修复的影响。通过将横断坐骨神经的近端和远端缝合到一个硅胶腔室中，构建了一个神经再生腔室。在远端切除3mm长的一段神经并将近端和远端残端插入腔室后，腔室内留下6mm的间隙。动物进行双侧手术，腔室的一侧填充1mg/ml的NGF/生理盐水（NS）（实验组），对侧填充NS（对照组）。术后1至5周，解剖并固定硅胶腔内的再生神经，用于光镜和超微结构水平的组织学研究。基于再生神经干直径更大、轴突数量更多以及髓鞘更厚，NGF腔室显示出更成熟的再生神经。
