在大鼠味蕾中表达的两种GTP结合蛋白α亚基，即Gα15和Gαq的鉴定。

Identification of two alpha-subunit species of GTP-binding proteins, Galpha15 and Galphaq, expressed in rat taste buds.

作者信息

Kusakabe Y, Yamaguchi E, Tanemura K, Kameyama K, Chiba N, Arai S, Emori Y, Abe K

机构信息

Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

出版信息

Biochim Biophys Acta. 1998 Jul 24;1403(3):265-72. doi: 10.1016/s0167-4889(98)00062-7.

DOI:10.1016/s0167-4889(98)00062-7

PMID:9685675

Abstract

We cloned cDNAs for two G protein alpha-subunits belonging to the Galphaq family, each capable of activating PLCbeta, from rat tongue. One is a Galphaq in the narrow sense, and the other, termed rat Galpha15, is a rat counterpart of mouse Galpha15, sharing an amino acid sequence similarity of 94%. RT-PCR and Northern blot analysis demonstrated that rat Galpha15 and Galphaq were distinctly expressed in tongue epithelia containing taste buds. Immunostaining also showed that rat Galpha15, together with the Galphaq, was localized mainly in taste buds. These studies suggest the possibility that these two Galpha proteins function for taste signal transduction in sensory cells.

摘要

我们从大鼠舌中克隆了两个属于Gαq家族的G蛋白α亚基的cDNA，每个亚基都能够激活磷脂酶Cβ（PLCβ）。一个是狭义上的Gαq，另一个称为大鼠Gα15，它是小鼠Gα15的大鼠对应物，氨基酸序列相似度为94%。逆转录聚合酶链反应（RT-PCR）和Northern印迹分析表明，大鼠Gα15和Gαq在含有味蕾的舌上皮中明显表达。免疫染色还显示，大鼠Gα15与Gαq一起主要定位于味蕾中。这些研究表明，这两种Gα蛋白可能在感觉细胞中参与味觉信号转导。