Han D K, Park K D, Hubbell J A, Kim Y H
Biomaterials Research Center, Korea Institute of Science and Technology, Seoul, Korea.
J Biomater Sci Polym Ed. 1998;9(7):667-80. doi: 10.1163/156856298x00082.
Novel lactide-based poly(ethylene glycol) (PEG) polymer networks (GL9-PEGs) were prepared by UV copolymerization of a glycerol-lactide triacrylate (GL9-Ac) with PEG monoacrylate (PEG-Ac) to use as scaffolds in tissue engineering, and the surface properties and biocompatibility of these networks were investigated as a function of PEG molecular weight and content. Analysis by ATR-FTIR and ESCA revealed that PEG was incorporated well within the GL9-PEG polymer networks and was enriched at the surfaces. From the results of SEM, AFM, and contact angle analyses, GL9-PEG networks showed relatively rough and irregular surfaces compared to GL9 network, but the mobile PEG chains coupled at their termini were readily exposed toward the aqueous environment when contacting water such that the surfaces became smoother and more hydrophilic. This reorientation and increase in hydrophilicity were more extensive with increasing PEG molecular weight and content. As compared to GL9 network lacking PEG, protein adsorption as well as platelet and S. epidermidis adhesion to GL9-PEG networks were significantly reduced as the molecular weight and content of PEG was increased, indicating that GL9-PEG networks are more biocompatible than the GL9 network due to PEG's passivity. Based on the physical and biological characterization reported, the GL9-PEG materials would appear to be interesting candidates as matrices for tissue engineering.
通过甘油 - 丙交酯三丙烯酸酯（GL9 - Ac）与聚乙二醇单丙烯酸酯（PEG - Ac）的紫外光共聚合制备了新型丙交酯基聚（乙二醇）（PEG）聚合物网络（GL9 - PEGs），用作组织工程中的支架，并研究了这些网络的表面性质和生物相容性与PEG分子量和含量的关系。ATR - FTIR和ESCA分析表明，PEG很好地掺入GL9 - PEG聚合物网络中并在表面富集。从扫描电子显微镜（SEM）、原子力显微镜（AFM）和接触角分析结果来看，与GL9网络相比，GL9 - PEG网络表面相对粗糙且不规则，但连接在其末端的可移动PEG链在接触水时容易暴露于水环境中，使得表面变得更光滑且更具亲水性。随着PEG分子量和含量的增加，这种重新取向和亲水性的增加更为显著。与缺乏PEG的GL9网络相比，随着PEG分子量和含量的增加，蛋白质吸附以及血小板和表皮葡萄球菌在GL9 - PEG网络上的粘附显著减少，这表明由于PEG的惰性，GL9 - PEG网络比GL9网络具有更好的生物相容性。基于所报道的物理和生物学特性，GL9 - PEG材料似乎是组织工程基质的有趣候选材料。
