Rajabnia Ramazan, Ghasempour Maryam, Gharekhani Samane, Gholamhoseinnia Sepide, Soroorhomayoon Sepide
Department of Microbiology, Babol University of Medical Sciences, Babol, Iran.
Department of Microbiology, Dental Material Research Center, Babol, Iran.
J Int Soc Prev Community Dent. 2016 Jan-Feb;6(1):49-53. doi: 10.4103/2231-0762.175405.
This study sought to assess the inhibitory effect of chitosan-containing sealants against Streptococcus mutans.
The antibacterial activity of the resin sealant was evaluated by direct contact test following the addition of 0, 1, 2, 3, 4, and 5 wt% chitosan. At 3, 6, 9, 24 and 48 h, 1 and 3 months, 10 μl of the microbial suspension in contact with resin sealant was cultured to count the number of colonies. Data were analyzed by one-way one-way analysis of variance (ANOVA), repeated measures ANOVA, and Scheffe test.
The minimum inhibitory concentration of chitosan against S. mutans was 2 wt%. At 3 h, bacterial count in the presence of 2-5 wt% chitosan was significantly lower than that at 0 and 1 wt% (P < 0.05). However, this difference in bacterial count between 2 and 3 wt% chitosan and between 4 and 5 wt% chitosan was not significant. At 6 h, the difference in bacterial count between 3 and 4 wt% chitosan was not significant, whereas the remaining groups were significantly different in terms of bacterial count at this time (P < 0.05). At the remaining time points, significant differences were found between 2 wt% chitosan and higher concentrations (P < 0.05).
Sealants containing 2-5 wt% chitosan show an antimicrobial property that is intensified by increasing the concentration of chitosan.
本研究旨在评估含壳聚糖封闭剂对变形链球菌的抑制作用。
在添加0、1、2、3、4和5 wt%壳聚糖后，通过直接接触试验评估树脂封闭剂的抗菌活性。在3、6、9、24和48小时、1个月和3个月时，对与树脂封闭剂接触的10 μl微生物悬液进行培养，以计算菌落数。数据采用单向方差分析（ANOVA）、重复测量方差分析和谢费检验进行分析。
壳聚糖对变形链球菌的最低抑菌浓度为2 wt%。在3小时时，2 - 5 wt%壳聚糖存在下的细菌计数显著低于0和1 wt%时的细菌计数（P < 0.05）。然而，2和3 wt%壳聚糖之间以及4和5 wt%壳聚糖之间的细菌计数差异不显著。在6小时时，3和4 wt%壳聚糖之间的细菌计数差异不显著，而此时其余组在细菌计数方面存在显著差异（P < 0.05）。在其余时间点，2 wt%壳聚糖与更高浓度之间存在显著差异（P < 0.05）。
含有2 - 5 wt%壳聚糖的封闭剂具有抗菌性能，且随着壳聚糖浓度的增加而增强。
