Koktysz M, Kieszko R, Dmoszyńska A, Milanowski J, Roliński J
Kliniki Hematologii AM w Lublinie.
Pol Arch Med Wewn. 1998 Jan;99(1):9-14.
Interleukin 2 (IL-2) and its receptor (IL-2R) plays the important role in the lymphocytic alveolitis in sarcoidosis. The aim of this study was the comparative analysis of expression of IL-2 receptor subunits: alpha chain (IL-2R alpha) and beta chain (IL-2R beta) in peripheral blood and broncho-alveolar lavage fluid (BAL) in patients with sarcoidosis. We examined 18 patients (aged 23 to 47 years, 8 women and 10 men) with sarcoidosis and 5 healthy persons (the control group consisted of 2 women and 3 men, aged 25 to 39 years). The expression of IL-2R was evaluated by immunophenotyping in ORTHO Cytoron Absolute flow cytometer. Comparing the patients group to the control group, we found in BAL fluid of sarcoidosis patients statistically higher number of lymphocytes with IL-2R alpha and IL-2R beta expression (p < 0.002), when the percentage of these lymphocytes was similar in both groups. In peripheral blood of sarcoidosis patients comparing to control group, we noted higher percentage of lymphocytes with expression of IL-2R alpha and IL-2R beta (more evident difference concerned IL-2R beta) but without statistical significance. We also compared BAL fluid to peripheral blood in patients group. We found in BAL fluid lower percentage of lymphocytes with expression IL-2R beta (p < 0.02) and no significant changes in percentage and number of lymphocytes IL-2R alpha+. Our data concerning IL-2 and IL-2R suggest the activation of T lymphocytes in the lung in sarcoidosis. They present only little reflection of immunological reactions, which take place in sarcoidosis infant. The above study should be completed in future by evaluation of the other activation markers including IL-2R gamma.
白细胞介素2（IL-2）及其受体（IL-2R）在结节病的淋巴细胞性肺泡炎中起重要作用。本研究的目的是比较结节病患者外周血和支气管肺泡灌洗液（BAL）中IL-2受体亚单位：α链（IL-2Rα）和β链（IL-2Rβ）的表达。我们检查了18例结节病患者（年龄23至47岁，8名女性和10名男性）和5名健康人（对照组由2名女性和3名男性组成，年龄25至39岁）。通过在ORTHO Cytoron Absolute流式细胞仪上进行免疫表型分析来评估IL-2R的表达。将患者组与对照组进行比较，我们发现在结节病患者的BAL液中，表达IL-2Rα和IL-2Rβ的淋巴细胞数量在统计学上显著更高（p < 0.002），而两组中这些淋巴细胞的百分比相似。与对照组相比，在结节病患者的外周血中，我们注意到表达IL-2Rα和IL-2Rβ的淋巴细胞百分比更高（与IL-2Rβ相关的差异更明显），但无统计学意义。我们还在患者组中比较了BAL液和外周血。我们发现在BAL液中表达IL-2Rβ的淋巴细胞百分比更低（p < 0.02），并且IL-2Rα +淋巴细胞的百分比和数量没有显著变化。我们关于IL-2和IL-2R的数据表明结节病患者肺部T淋巴细胞被激活。它们仅很少反映结节病婴儿中发生的免疫反应。上述研究未来应通过评估包括IL-2Rγ在内的其他激活标志物来完成。
