Willis C M, Reiche L, Wilkinson J D
Department of Dermatology, Amersham Hospital, Bucks, UK.
Contact Dermatitis. 1998 Jul;39(1):21-7. doi: 10.1111/j.1600-0536.1998.tb05806.x.
The aims of this study were to utilize quantitative immunocytochemical techniques to determine the densities of keratin 16 (K16) and keratin 17 (K17) expressed by keratinocytes during the course of acute patch test reactions to sodium lauryl sulfate (SLS), and to relate these to the proliferative state of the epidermis, as assessed by Ki-67 immunolabelling. Significantly increased numbers of dividing keratinocytes were present in 48h and 96h reactions, concurrent with high levels of expression of K16 and more moderate expression of K17. Statistical analysis indicated a good correlation between K16 expression and the density of Ki-67+ keratinocytes present in the epidermis (r=0.843). This was not the case for K17 (r=0.396). The results demonstrate that both K16 and K17 expression are features of acute irritant contact dermatitis reactions, but suggest that the factors which influence and control their expression differ.
本研究的目的是利用定量免疫细胞化学技术，确定在对月桂醇硫酸酯钠（SLS）进行急性斑贴试验反应过程中角质形成细胞表达的角蛋白16（K16）和角蛋白17（K17）的密度，并将这些与通过Ki-67免疫标记评估的表皮增殖状态相关联。在48小时和96小时的反应中，存在显著增多的正在分裂的角质形成细胞，同时伴有K16的高表达和K17的中度表达。统计分析表明，K16表达与表皮中Ki-67+角质形成细胞的密度之间存在良好的相关性（r = 0.843）。K17的情况并非如此（r = 0.396）。结果表明，K16和K17的表达都是急性刺激性接触性皮炎反应的特征，但提示影响和控制它们表达的因素有所不同。
