Flouriot G, Ducouret B, Byrnes L, Valotaire Y
Endocrinologie Moléculaire de la Reproduction, UPRES-A Biologie Cellulaire et Reproduction, INRA, Université de Rennes I, France.
J Mol Endocrinol. 1998 Jun;20(3):355-62. doi: 10.1677/jme.0.0200355.
Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene is also regulated by this hormone. Indeed, treatment of hepatocytes with 1 microM estradiol led, after 24 h, to a dramatic decrease in Alb mRNA level. To investigate the mechanism of this down-regulation, run-off experiments were performed and mRNA half-lives were determined in the presence and absence of estradiol. The results show that the down-regulation of Alb mRNA expression by estrogens occurs only at the transcriptional level.
雌激素可调节卵生动物中许多肝脏特异性基因的表达。例如，在虹鳟鱼肝中，雌激素受体和卵黄蛋白原基因的表达会被雌二醇强烈上调。利用肝细胞原代培养，我们证明鳟鱼白蛋白（Alb）基因也受这种激素调节。实际上，用1微摩尔/升的雌二醇处理肝细胞24小时后，Alb mRNA水平显著下降。为了研究这种下调的机制，进行了连续转录实验，并在有或没有雌二醇的情况下测定了mRNA半衰期。结果表明，雌激素对Alb mRNA表达的下调仅发生在转录水平。
