施氏假单胞菌反硝化过程中对RpoN（σ因子σ54）的需求是间接的，且仅限于亚硝酸盐和一氧化氮的还原。
The requirement of RpoN (sigma factor sigma54) in denitrification by Pseudomonas stutzeri is indirect and restricted to the reduction of nitrite and nitric oxide.
作者信息
Härtig E, Zumft W G
机构信息
Lehrstuhl für Mikrobiologie der Universität Karlsruhe, D-76128 Karlsruhe, Germany.
出版信息
Appl Environ Microbiol. 1998 Aug;64(8):3092-5. doi: 10.1128/AEM.64.8.3092-3095.1998.
Abstract
The rpoN region of Pseudomonas stutzeri was cloned, and an rpoN null mutant was constructed. RpoN was not essential for denitrification in this bacterium but affected the expression levels and enzymatic activities of cytochrome cd1 nitrite reductase and nitric oxide reductase, whereas those of respiratory nitrate reductase and nitrous oxide reductase were comparable to wild-type levels. Since the transcription of the structural genes nirS and norCB, coding for nitrite reductase and the nitric oxide reductase complex, respectively, proceeded unabated, our data indicate a posttranslational process for the two key enzymes of denitrification depending on RpoN.
摘要
我们克隆了施氏假单胞菌的rpoN区域，并构建了rpoN基因缺失突变体。RpoN对该细菌的反硝化作用并非必不可少，但它影响了细胞色素cd1亚硝酸还原酶和一氧化氮还原酶的表达水平及酶活性，而呼吸性硝酸盐还原酶和氧化亚氮还原酶的表达水平与野生型相当。由于分别编码亚硝酸还原酶和一氧化氮还原酶复合物的结构基因nirS和norCB的转录不受影响，我们的数据表明反硝化作用的这两种关键酶存在依赖于RpoN的翻译后加工过程。
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