Warren K S, Fishman M C
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA.
Am J Physiol. 1998 Jul;275(1):H1-7. doi: 10.1152/ajpheart.1998.275.1.H1.
Large-scale mutagenesis screens have proved essential in the search for genes that are important to development in the fly, worm, and yeast. Here we present the power of large-scale screening in a vertebrate, the zebrafish Danio rerio, and propose the use of this genetic system to address fundamental questions of vertebrate developmental physiology. As an example, we focus on zebrafish mutations that reveal single genes essential for normal development of the cardiovascular system. These single gene mutations disrupt specific aspects of rate, rhythm, conduction, or contractility of the developing heart.
大规模诱变筛选已被证明在寻找对果蝇、线虫和酵母发育至关重要的基因方面必不可少。在此，我们展示了在脊椎动物斑马鱼（Danio rerio）中进行大规模筛选的作用，并提议利用这个遗传系统来解决脊椎动物发育生理学的基本问题。作为一个例子，我们重点关注那些揭示对心血管系统正常发育至关重要的单个基因的斑马鱼突变。这些单基因突变会破坏发育中心脏的速率、节律、传导或收缩性等特定方面。
