Gaubatz S, Wood J G, Livingston D M
Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA.
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9190-5. doi: 10.1073/pnas.95.16.9190.
E2F transcription factors play an important role in the regulation of cell cycle progression. We report here the cloning and characterization of an additional member of this family, E2F-6. E2F-6 lacks pocket protein binding and transactivation domains, and it is a potent transcriptional repressor that contains a modular repression domain at its carboxyl terminus. Overproduction of E2F-6 had no specific effect on cell cycle progression in asynchronously growing Saos2 and NIH 3T3 cells, but it inhibited entry into S phase of NIH 3T3 cells stimulated to exit G0. Taken together, these data suggest that E2F-6 can regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression.
E2F转录因子在细胞周期进程的调控中发挥重要作用。我们在此报告该家族另一个成员E2F-6的克隆及特性。E2F-6缺乏口袋蛋白结合域和反式激活域，是一种强效转录抑制因子，在其羧基末端含有一个模块化抑制域。在异步生长的Saos2和NIH 3T3细胞中，过量表达E2F-6对细胞周期进程没有特定影响，但它抑制了被刺激退出G0期的NIH 3T3细胞进入S期。综上所述，这些数据表明E2F-6可调控一部分E2F依赖性基因，这些基因的产物是进入细胞周期所必需的，但对正常细胞周期进程并非必需。
