Gattuso J M, Debinski H S, Kangro H O, Jeffries D, Kamm M A
St. Mark's Hospital, Harrow, Middlesex, UK.
Int J Colorectal Dis. 1998;13(3):131-3. doi: 10.1007/s003840050150.
The aetiology of idiopathic megarectum and idiopathic megacolon is unknown. A previous study in patients with chronic idiopathic intestinal pseudo-obstruction, a condition also associated with a dilated gut, identified the possible involvement of herpes viruses. This study therefore aimed to determine whether these viruses may also be implicated in the pathogenesis of these conditions.
Resected large bowel from three patients with idiopathic megarectum and three patients with idiopathic megacolon were studied. Histology for viral inclusions and nested polymerase chain reaction (PCR) using specific primers for cytomegalovirus, Epstein-Barr virus, herpes simplex virus type 1 and varicella zoster virus was performed. DNA was extracted from paraffin-embedded blocks by proteinase K and phenol chloroform extraction.
Viral inclusions were not seen. PCR failed to identify DNA of the four herpes viruses tested.
Patients with idiopathic megarectum or idiopathic megacolon may have subtle abnormalities of the enteric innervation, but these do not appear to be attributable to the neurotropic effects of the herpes viruses studied.
特发性巨直肠和特发性巨结肠的病因尚不清楚。先前一项针对慢性特发性肠道假性梗阻患者(一种同样与肠道扩张相关的病症)的研究发现,疱疹病毒可能参与其中。因此,本研究旨在确定这些病毒是否也与这些病症的发病机制有关。
对3例特发性巨直肠患者和3例特发性巨结肠患者切除的大肠进行研究。进行了病毒包涵体组织学检查,并使用针对巨细胞病毒、爱泼斯坦 - 巴尔病毒、1型单纯疱疹病毒和水痘 - 带状疱疹病毒的特异性引物进行巢式聚合酶链反应(PCR)。通过蛋白酶K和酚氯仿提取法从石蜡包埋块中提取DNA。
未发现病毒包涵体。PCR未能鉴定出所检测的四种疱疹病毒的DNA。
特发性巨直肠或特发性巨结肠患者的肠道神经支配可能存在细微异常,但这些异常似乎并非由所研究的疱疹病毒的嗜神经作用所致。