King G N, King N, Hughes F J
Department of Periodontology, St Bartholomew's & The Royal London School of Medicine and Dentistry, University of London, UK.
J Periodontal Res. 1998 May;33(4):226-36. doi: 10.1111/j.1600-0765.1998.tb02194.x.
Resorbable collagen membranes for guided tissue regeneration in periodontal therapy have shown promise but are not osteoinductive. As recombinant human bone morphogenetic protein-2 (rhBMP-2) is known to have an affinity for collagen, the use of this osteoinductive agent incorporated into a collagen vehicle may act as a suitable carrier to promote periodontal regeneration. The aim of this study was to investigate the effects of two different collagen delivery systems for rhBMP-2 in rat periodontal fenestration defects. Using the collagen membrane delivery system, 3 groups of adult Wistar rats which had surgical defects created on the right side of the mandible involving the removal of bone and exposure of the molar roots were treated with either rhBMP-2 in colagen membrane (BMPm) (n = 12 animals), or collagen membrane only (COLm) (n = 12), or were left untreated (UN) (n = 14). Using the collagen gel delivery system, surgical defects were treated with either rhBMP-2 incorporated in a collagen gel carrier (BMPg) (n = 5) or had collagen gel only (COLg) (n = 6). Animals were killed 10 d postoperatively and tissues processed for histology. New bone formation was significantly greater in BMPg compared with both BMPm and controls (p < 0.05). However, new cementum formation was significantly greater in BMPm (721 +/- 166 micron2, mean +/- SE) compared with COLm, COLg and UN (p < 0.02) (190 +/- 44 micron2, 327 +/- 114 micron2 and 172 +/- 33 micron2, respectively) and more than 1.5 times BMPg (451 +/- 158 micron2). In conclusion, both carrier systems for rhBMP-2 significantly increased new bone formation compared with controls during the early stages of periodontal wound healing. However, the more slowly dissolving collagen membrane carrier system for rhBMP-2 produced significantly greater new cementum compared with the collagen gel carrier, suggesting that a more prolonged exposure of rhBMP-2 is required to increased cementogenesis.
用于牙周治疗引导组织再生的可吸收胶原膜已显示出前景，但不具有骨诱导性。由于重组人骨形态发生蛋白-2（rhBMP-2）已知对胶原具有亲和力，将这种骨诱导剂掺入胶原载体中使用可能作为促进牙周再生的合适载体。本研究的目的是研究两种不同的rhBMP-2胶原递送系统对大鼠牙周开窗缺损的影响。使用胶原膜递送系统，3组成年Wistar大鼠在下颌右侧制造手术缺损，包括去除骨组织并暴露磨牙牙根，分别用胶原膜中的rhBMP-2（BMPm）（n = 12只动物）、仅胶原膜（COLm）（n = 12）处理，或不进行处理（UN）（n = 14）。使用胶原凝胶递送系统，手术缺损分别用掺入胶原凝胶载体中的rhBMP-2（BMPg）（n = 5）或仅用胶原凝胶（COLg）（n = 6）处理。术后10天处死动物并对组织进行组织学处理。与BMPm和对照组相比，BMPg中的新骨形成明显更多（p < 0.05）。然而，与COLm、COLg和UN相比，BMPm中的新牙骨质形成明显更多（721 +/- 166平方微米，平均值 +/- 标准误）（分别为190 +/- 44平方微米、327 +/- 114平方微米和172 +/- 33平方微米）（p < 0.02），并且是BMPg（451 +/- 158平方微米）的1.5倍以上。总之，与对照组相比，两种rhBMP-2载体系统在牙周伤口愈合早期均显著增加了新骨形成。然而，rhBMP-2的溶解较慢的胶原膜载体系统与胶原凝胶载体相比产生了明显更多的新牙骨质，这表明需要更长时间暴露rhBMP-2以增加牙骨质生成。
