Soedarmanto I, Zhicai W, Setyamahanani A, Lämmler C
Institut für Bakteriologie und Immunologie, Giessen, Germany.
Res Vet Sci. 1998 May-Jun;64(3):181-5. doi: 10.1016/s0034-5288(98)90121-7.
The present study was designed to comparatively investigate 21 Rhodococcus equi isolates from the faeces of clinically healthy horses and cattle. The isolates were identified by cultural and biochemical properties and by PCR analysis. The latter, targeted to the gene coding for the 16S ribosomal RNA, revealed a species specific PCR product. The isolates were further characterised by serotyping with two typing systems, by haemagglutination tests and by plasmid and virulence protein profiling. Among the 21 cultures, four cultures contained plasmids, two of the four cultures expressed 15-17 kDa virulence proteins, no cultures contained 20 kDA virulence proteins. The 21 cultures were further analysed by DNA-fingerprinting. This was performed by macrorestriction analysis of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). The DNA-restriction patterns were different for most of the isolates indicating a clone heterogeneity among isolates from single farms. Serotyping, determination of virulence marker and PFGE analysis of R equi appeared to be useful for further characterisation of this species, possibly of importance for virulence estimation of single R equi isolates and for epidemiological studies.
本研究旨在比较调查从临床健康马匹和牛粪便中分离出的21株马红球菌。通过培养和生化特性以及PCR分析对分离株进行鉴定。后者针对编码16S核糖体RNA的基因，揭示了一种物种特异性PCR产物。通过两种分型系统进行血清分型、血凝试验以及质粒和毒力蛋白分析，对分离株进行进一步表征。在这21个培养物中，有4个培养物含有质粒，4个培养物中的2个表达15 - 17 kDa的毒力蛋白，没有培养物含有20 kDa的毒力蛋白。通过DNA指纹分析对这21个培养物进行进一步分析。这是通过脉冲场凝胶电泳（PFGE）对染色体DNA进行宏观限制性分析来完成的。大多数分离株的DNA限制性图谱不同，表明来自单个农场的分离株之间存在克隆异质性。马红球菌的血清分型、毒力标记物测定和PFGE分析似乎有助于对该物种进行进一步表征，这可能对单个马红球菌分离株的毒力评估和流行病学研究具有重要意义。
