Soedarmanto I, Oliveira R, Lämmler C, Dürrling H
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.
Zentralbl Bakteriol. 1997 Nov;286(4):457-67. doi: 10.1016/s0934-8840(97)80047-3.
The present study was designed to comparatively investigate 10 Rhodococcus equi isolates from cases of lymphadenitis in cattle. The isolates could be identified by cultural and biochemical properties. By serotyping the R. equi isolates 9 and 1, cultures could be classified as Nakazawa's serotypes 15 and 8, respectively. The isolates did not agglutinate rabbit erythrocytes, were uniformly susceptible to most of the antibiotics tested, did not contain plasmids nor expressed virulence-associated proteins and yielded identical patterns in protein fingerprinting. To further analyze the epidemiological relationships, the isolates were additionally subjected to DNA fingerprinting. This was performed by pulsed-field gel electrophoresis (PFGE) after digestion of the chromosomal DNA with the endonuclease AsnI. PFGE analysis of the chromosomal DNA revealed 4 DNA restriction groups with DNA pattern I with 7 isolates as predominant group and DNA pattern II to IV with one isolate, respectively. The present results indicate that a single R. equi clone belonging to Nakazawa's serotype 15 and according to PFGE to DNA restriction pattern I of the present investigation seems to be responsible for most of the cases of lymphadenitis of cattle described in this study.
本研究旨在比较调查从牛淋巴结炎病例中分离出的10株马红球菌。这些分离株可通过培养和生化特性进行鉴定。通过对马红球菌分离株进行血清分型，分离株9和1的培养物可分别归类为中泽血清型15和8。这些分离株不凝集兔红细胞，对大多数测试抗生素均普遍敏感，不含有质粒，也不表达毒力相关蛋白，并且在蛋白质指纹图谱中产生相同的模式。为了进一步分析流行病学关系，对这些分离株还进行了DNA指纹图谱分析。这是在用内切酶AsnI消化染色体DNA后，通过脉冲场凝胶电泳（PFGE）进行的。对染色体DNA的PFGE分析揭示了4个DNA限制组，其中DNA模式I有7个分离株为主要组，DNA模式II至IV各有1个分离株。目前的结果表明，本研究中描述的大多数牛淋巴结炎病例似乎是由属于中泽血清型15且根据PFGE属于本研究DNA限制模式I的单一马红球菌克隆引起的。
