Otto B, Ogilvie A
Biochemical Institute, Faculty of Medicine, Friedrich-Alexander-University, Erlangen, Germany.
Biomaterials. 1998 Jun;19(11-12):1049-55. doi: 10.1016/s0142-9612(98)00028-3.
The bioactive calcium phosphate ceramics with various calcium: phosphorus ratios: Ca/P = 1.67 (hydroxyapatite, HA), Ca/P = 1.6 and Ca/P = 1.5 (tricalcium phosphate, beta-TCP), the bioinert aluminium oxide ceramic (Al2O3) and the toxic calcium oxide ceramic (CaO) have been investigated with respect to their ability to activate peritoneal macrophages of NMRI-mice and with respect to their influence on the extracellular nucleotide degradation of these macrophages. Two weeks after the intraperitoneal injection of a suspension of ceramic particles in an isotone salt solution (phosphate-buffered saline = PBS), we observed that the peritoneal macrophages were only slightly activated into the responsive state, independent of the type of ceramic. 5'Nucleotidase (5'N) ectoenzyme hydrolyses adenosine monophosphate (AMP) and a decrease of its activity is a general biochemical marker of activated macrophages. This ectoenzyme activity was slightly reduced after ceramic implantation. The lacking rise of the extracellular diadenosine tetraphosphate (Ap4A)-catabolism by the macrophage ectoenzyme alkaline phosphodiesterase I (APD) demonstrated that the peritoneal macrophages did not completely reach the responsive state. After the implantation of calcium phosphate ceramics the extracellular adenosine triphosphate (ATP)-reduction was slightly diminished. After the implantation of tricalcium phosphate ceramic about 30% more peritoneal exsudate cells (PEC) were obtained from the peritoneal cavity than after injections of pure PBS (used as non-inflammatory control). Similar to the phenomena following the injection of thioglycollate (Tg, inflammation producing control agent) a slightly but not significantly increased proportion of pseudopodia-building cells was observed after the implantation of the ceramic with Ca/P = 1.6.
对具有不同钙磷比的生物活性磷酸钙陶瓷（钙磷比Ca/P = 1.67，即羟基磷灰石，HA；Ca/P = 1.6；Ca/P = 1.5，即β-磷酸三钙，β-TCP）、生物惰性氧化铝陶瓷（Al₂O₃）以及有毒的氧化钙陶瓷（CaO），研究了它们激活NMRI小鼠腹膜巨噬细胞的能力以及对这些巨噬细胞细胞外核苷酸降解的影响。在腹腔注射陶瓷颗粒悬浮液于等渗盐溶液（磷酸盐缓冲盐水 = PBS）两周后，我们观察到，无论陶瓷类型如何，腹膜巨噬细胞仅被轻微激活至反应状态。5'-核苷酸酶（5'N）这种胞外酶可水解单磷酸腺苷（AMP），其活性降低是活化巨噬细胞的一个通用生化标志物。陶瓷植入后，这种胞外酶活性略有降低。巨噬细胞胞外酶碱性磷酸二酯酶I（APD）介导的细胞外四磷酸二腺苷（Ap4A）分解代谢缺乏升高，这表明腹膜巨噬细胞并未完全达到反应状态。植入磷酸钙陶瓷后，细胞外三磷酸腺苷（ATP）的减少略有减弱。植入磷酸三钙陶瓷后，从腹腔获得的腹膜渗出细胞（PEC）比注射纯PBS（用作非炎性对照）后多约30%。与注射巯基乙酸盐（Tg，炎症诱导对照剂）后的现象类似，植入钙磷比Ca/P = 1.6的陶瓷后，观察到伪足形成细胞的比例略有增加，但无统计学意义。
