Ross L, Benghuzzi H, Tucci M, Callender M, Cason Z, Spence L
School of Health Related Professions, University of Mississippi Medical Center, Jackson 39216, USA.
Biomed Sci Instrum. 1996;32:71-9.
The relationship between various bioceramics used in surgical implantation and inflammatory cellular response has not been fully elucidated. The objective of this study was to investigate the effect of various biomedical ceramics such as tricalcium phosphate (TCP), hydroxyapatite (HA), and aluminum-calcium-phosphorous oxide (ALCAP) on the adherence and viability of human monocyte and monocyte derived macrophages in vitro. The monocytes were isolated from human peripheral blood and seeded at a density of 5 x 10(5) cells/well according to standard laboratory procedures. Cells were considered macrophages after remaining in culture for 24 hours. Cells were then plated in each microtiter well loaded with ceramic capsules (HA, TCP and ALCAP) and buffered control. At the end of 1, 2, 3, and 7 days the viability and cell number of monocyte or monocyte derived macrophages were determined using an established assay. Cell number was determined in control wells with known amounts of cell number, a standard curve was generated by plotting absorbance units versus cell number. Biochemical analysis was performed on the aliquots obtained from the experimental and control wells at the end of each phase of the investigation. The data from this experiment suggest that: (I) monocytes and macrophages are capable of adhering to the surface of HA, TCP and ALCAP in an in vitro environment for over a 7 day period. (II) Long term incubation of ceramic capsules with macrophages revealed that the cells experienced gradual disassociation phenomenon with a greater number of cell detachment seen in the ALCAP contained wells. (III) SEM analysis of representative capsules demonstrated that there is an increase in the number of micropores on the surface of the materials after contacting a cellular environment. This observation suggest that the material surface has been modified (TCP > HA = ALCAP). (IV) Biochemical analysis of aliquots at the end of each phase showed a significant change (P < 0.05) in the activity of catalase and superoxide dismutase (SOD). Information obtained from this study provided new insights on the interrelationship between bioceramics and the possible cell response during chronic inflammation at the site of implantation.
用于外科植入的各种生物陶瓷与炎症细胞反应之间的关系尚未完全阐明。本研究的目的是调查各种生物医学陶瓷，如磷酸三钙（TCP）、羟基磷灰石（HA）和铝钙磷氧化物（ALCAP）对人单核细胞和单核细胞衍生巨噬细胞在体外的黏附及活力的影响。单核细胞从人外周血中分离出来，并按照标准实验室程序以5×10⁵个细胞/孔的密度接种。细胞在培养24小时后被视为巨噬细胞。然后将细胞接种到每个装有陶瓷胶囊（HA、TCP和ALCAP）和缓冲对照的微量滴定孔中。在1、2、3和7天结束时，使用既定的检测方法测定单核细胞或单核细胞衍生巨噬细胞的活力和细胞数量。在已知细胞数量的对照孔中测定细胞数量，通过绘制吸光度单位与细胞数量的关系生成标准曲线。在研究的每个阶段结束时，对从实验孔和对照孔中获得的等分试样进行生化分析。该实验的数据表明：（I）单核细胞和巨噬细胞能够在体外环境中在HA、TCP和ALCAP表面黏附超过7天。（II）陶瓷胶囊与巨噬细胞的长期孵育显示，细胞出现逐渐解离现象，在含有ALCAP的孔中观察到更多的细胞脱落。（III）对代表性胶囊的扫描电子显微镜分析表明，在接触细胞环境后，材料表面的微孔数量增加。这一观察结果表明材料表面已被修饰（TCP>HA=ALCAP）。（IV）每个阶段结束时对等分试样的生化分析显示，过氧化氢酶和超氧化物歧化酶（SOD）的活性有显著变化（P<0.05）。从本研究中获得的信息为生物陶瓷与植入部位慢性炎症期间可能的细胞反应之间的相互关系提供了新的见解。
