Unson M D, Newton G L, Davis C, Fahey R C
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093, USA.
J Immunol Methods. 1998 May 1;214(1-2):29-39. doi: 10.1016/s0022-1759(98)00034-9.
Mycothiol (MSH) is a glycosylated derivative of N-acetylcysteine that may have antioxidant functions in mycobacteria and other actinomycetes. To develop a highly specific assay for MSH, we capitalized on the selective binding of thiols to a maleimide residue linked to bovine serum albumin and employed affinity-purified polyclonal antibody and an enzyme-linked secondary antibody for detection. The assay was shown to be specific and to detect MSH at levels as low as 0.1 pmol when conducted in the form of a microtiter plate-based ELISA. A similar, nitrocellulose membrane-based immunoassay was shown to be useful for qualitative detection of MSH-producing bacterial colonies.
巯基乙醇（MSH）是N - 乙酰半胱氨酸的糖基化衍生物，可能在分枝杆菌和其他放线菌中具有抗氧化功能。为了开发一种针对MSH的高特异性检测方法，我们利用硫醇与连接到牛血清白蛋白的马来酰亚胺残基的选择性结合，并使用亲和纯化的多克隆抗体和酶联二抗进行检测。当以基于微量滴定板的酶联免疫吸附测定（ELISA）形式进行时，该检测方法显示出特异性，并且能够检测低至0.1皮摩尔水平的MSH。一种类似的基于硝酸纤维素膜的免疫测定方法被证明可用于定性检测产生MSH的细菌菌落。
