Klimenko A I, Stefanovich A N
Vopr Pitan. 1976 May-Jun(1):15-18.
The sulfydryl and disulfide groups content in summary histones of the hepatic cell nuclei in the Wistar albino rats aged 1, 3, 12 and 24 months was studied by employing amperometric titration under normal conditions and under the action on the organism of calorifically substandard nutrition tending to significantly prolong the life-span in these animals. In intact animals the content of thiol groups in histones was found to reach the maximum on the 3d month of their life, to continue at this level during the first year and to decline in old (34 month old) animals. In the post-natal ontogenesis of the rats the ratio of sulfhydryl to disulfide groups in the histone proteins does not show any noticeable changes. With calorific deficiency this ratio increases in histones of old animals. The possible significance of the thiol groups balance in the histone proteins in regulating the genome activity during age-dependent development of the animals is discussed.
采用安培滴定法，研究了1、3、12和24月龄Wistar白化大鼠在正常条件下以及在热量不足营养作用下（这种营养倾向于显著延长这些动物的寿命）肝细胞核酸组蛋白中巯基和二硫键的含量。在未受影响的动物中，发现组蛋白中巯基含量在其生命的第3个月达到最高，在第一年保持这一水平，而在老年（24月龄）动物中下降。在大鼠出生后的个体发育过程中，组蛋白中巯基与二硫键的比例没有明显变化。热量不足时，老年动物组蛋白中的这一比例会增加。文中讨论了组蛋白中巯基平衡在动物年龄依赖性发育过程中调节基因组活性的可能意义。
