Clugston S L, Daub E, Honek J F
Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
J Mol Evol. 1998 Aug;47(2):230-4. doi: 10.1007/pl00006380.
Glyoxalase I (GlxI) is the first of two enzymes involved in the cellular detoxification of methylglyoxal. A recent search of the National Center for Biotechnology Information (NCBI) databases with the protein sequence of Salmonella typhimurium GlxI identified two new hypothetical proteins with unassigned function. These two sequences, from Brassica oleracea and Sporobolus stapfianus, have significant sequence similarity to known GlxI sequences, suggesting that these two open reading frames encode for GlxI in these plants. Interestingly, analysis of these two new sequences indicates that they code for a protein composed of two fused monomers, a situation previously found solely in the yeast GlxI enzymes.
乙二醛酶I（GlxI）是参与甲基乙二醛细胞解毒的两种酶中的第一种。最近，利用鼠伤寒沙门氏菌GlxI的蛋白质序列在国家生物技术信息中心（NCBI）数据库中进行搜索，发现了两个功能未明确的新假设蛋白质。这两个序列分别来自甘蓝和斯氏鼠尾粟，与已知的GlxI序列具有显著的序列相似性，表明这两个开放阅读框在这些植物中编码GlxI。有趣的是，对这两个新序列的分析表明，它们编码的蛋白质由两个融合的单体组成，这种情况以前仅在酵母乙二醛酶I中发现。
