[少精子症男性精子DNA含量的研究]
[Studies on DNA content in sperm of men with oligozoospermia].
作者信息
Skonieczna D, Kotwicka M, Filipiak K, Warchoł W, Fischer Z, Warchoł J B
机构信息
Zakładu Radiobiologii i Biologii Komórki Akademii Medycznej im. Karola Marcinkowskiego w Poznaniu.
出版信息
Ginekol Pol. 1998 Jun;69(6):517-23.
The aim of our study was to analysis the ploidy of spermatozoa from oligozoospermic individuals. The study was performed in 48 patients with oligozoospermia. Spermatozoal DNA was analysed using MagiCal imaging cytometry. The percentage of aneuploid cells was determined using CYT-2 program. The results indicated aneuploidy in 73% of the examined patients. The amount of cells with abnormal DNA content ranged from 30 to 100%. Monitoring of the DNA in human spermatozoa may have practical applications in establishing the reasons for infertility as well as for screening of the semen for assisted reproduction techniques.
我们研究的目的是分析少精子症患者精子的倍性。该研究在48例少精子症患者中进行。使用MagiCal成像细胞仪分析精子DNA。使用CYT - 2程序确定非整倍体细胞的百分比。结果表明，73%的受检患者存在非整倍体情况。DNA含量异常的细胞数量范围为30%至100%。监测人类精子中的DNA在确定不孕原因以及辅助生殖技术精液筛查方面可能具有实际应用价值。
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