Künkel W
Z Allg Mikrobiol. 1976;16(3):187-96. doi: 10.1002/jobm.3630160303.
Inducibility of alcohol dehydrogenase (ADH) crystals in yeast cells depends on the growth phase. They accumulate during exponential growth, a process that is parallelled by accumulation of a special protein (H protein). The following pieces of evidence show that crystals, H protein, and ADH are identical: (1) The three proteins have the same amino acid composition and subunit molecular weight; (2) active H protein and ADH exhibit the same electrophoretic mobility, indicating a common molecular weight of about 158 000 D; (3) the native H protein and a crystal fraction stabilized by Zn++ and dialyzed against EDTA possess ADH activity.
酵母细胞中乙醇脱氢酶（ADH）晶体的可诱导性取决于生长阶段。它们在指数生长期积累，这一过程伴随着一种特殊蛋白质（H蛋白）的积累。以下证据表明晶体、H蛋白和ADH是相同的：（1）这三种蛋白质具有相同的氨基酸组成和亚基分子量；（2）活性H蛋白和ADH表现出相同的电泳迁移率，表明其共同分子量约为158000道尔顿；（3）天然H蛋白和经Zn++稳定并经EDTA透析的晶体部分具有ADH活性。
