Furlanetto S, Gratteri P, Pinzauti S, Leardi R, Dreassi E, Santoni G
Dipartimento di Scienze Farmaceutiche, Università di Firenze, Italy.
J Pharm Biomed Anal. 1995 Apr;13(4-5):431-8. doi: 10.1016/0731-7085(95)01288-v.
An adsorptive stripping voltammetric method with a hanging mercury drop electrode was developed for the determination of the fluoroquinolone rufloxacin in tablets, human plasma and urine. Measurements were obtained in differential pulse mode and a multivariate strategy was used to optimize the variables involved. Besides the independent effects of the variables, a strong interaction between scan rate and pulse duration has been found. Rufloxacin was analysed at concentrations between 1.7 x 10(-8) and 1.9 x 10(-7) M with a detection limit of 9.2 x 10(-9) M. Diluted tablet solutions and urine samples were analysed directly, while plasma samples needed an extraction procedure before voltammetric analysis. An improved HPLC procedure was used as comparative method.
