差分脉冲吸附溶出伏安法测定抗菌药物洛美沙星
Differential-pulse adsorptive stripping voltammetric determination of the antibacterial lomefloxacin.
作者信息
Vílchez J L, Araujo L, Prieto A, Navalón A
机构信息
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/. Fuentenueva s/n, E-18071 Granada, Spain.
出版信息
J Pharm Biomed Anal. 2001 Aug;26(1):23-9. doi: 10.1016/s0731-7085(01)00391-0.
A differential-pulse adsorptive stripping voltammetric method for the determination of trace amounts of the antibacterial lomefloxacin is proposed. By using an accumulation potential of -0.30 V and a 2 min accumulation time, the linear concentration range of application was 1.0-10.0 ng ml(-1) of lomefloxacin, with a relative standard deviation of 3.8% (for a level of 5.0 ng ml(-1)) and a detection limit of 0.3 ng ml(-1). The method was applied to determination of lomefloxacin in human urine and serum samples. It was validated using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.
提出了一种差分脉冲吸附溶出伏安法测定痕量抗菌药物洛美沙星。采用-0.30 V的富集电位和2分钟的富集时间，洛美沙星的线性应用浓度范围为1.0 - 10.0 ng ml⁻¹，相对标准偏差为3.8%（对于5.0 ng ml⁻¹的水平），检测限为0.3 ng ml⁻¹。该方法应用于人体尿液和血清样品中洛美沙星的测定。以高效液相色谱法作为参考方法进行了验证。在所有情况下，该方法的回收率均达到100%。
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