Eriksson B M, Wallin A
Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
J Pharm Biomed Anal. 1995 Apr;13(4-5):551-61. doi: 10.1016/0731-7085(95)01259-n.
Three liquid-chromatography columns, containing immobilized proteins as chiral stationary phases (CSPs) were investigated for the direct separation of enantiomers of racemic indenoindolic compounds. By using an experimental design the effects of column temperature, pH, ionic strength, and type and concentration of organic solvent in the mobile phase on retention and resolution of racemic substances were systematically studied. The three CSPs investigated consisted of alpha(1)-acid glycoprotein (Chiral-AGP), bovine serum albumin (BSA-DSC) and ovomucoid (Ultron ES-OVM). The two enantiomers of all studied compounds could be separated on at least one of the three CSPs, which have different enantioselective properties.
研究了三根含有固定化蛋白质作为手性固定相（CSPs）的液相色谱柱，用于直接分离外消旋茚并吲哚化合物的对映体。通过实验设计，系统地研究了柱温、pH值、离子强度以及流动相中有机溶剂的类型和浓度对外消旋物质保留和分离度的影响。所研究的三种CSPs分别由α(1)-酸性糖蛋白（Chiral-AGP）、牛血清白蛋白（BSA-DSC）和卵类粘蛋白（Ultron ES-OVM）组成。所有研究化合物的两种对映体均可在具有不同对映选择性的三种CSPs中的至少一种上实现分离。
