Institute for Biosciences, Mukogawa Women's University.
Chem Pharm Bull (Tokyo). 2022;70(7):458-468. doi: 10.1248/cpb.c22-00269.
A lot of chiral stationary phases (CSPs) have been introduced for the purpose of analytical and preparative separations of enantiomers. CSPs based on proteins and glycoproteins have unique properties among those CSPs. This review article deals with the preparation of CSPs based on proteins and glycoproteins, their chiral recognition properties and mechanisms, focusing on the CSPs investigated in our group. The dealt proteins and glycoproteins are including bovine serum albumin, human serum albumin, lysozyme, pepsin, human α-acid glycoprotein (AGP), chicken ovomucoid and chicken ovoglycoprotein (named chicken AGP).
已经引入了许多手性固定相 (CSP) 来进行对映异构体的分析和制备分离。在这些 CSP 中，基于蛋白质和糖蛋白的 CSP 具有独特的性质。本文综述了基于蛋白质和糖蛋白的 CSP 的制备、手性识别特性和机制，重点介绍了我们小组研究的 CSP。所涉及的蛋白质和糖蛋白包括牛血清白蛋白、人血清白蛋白、溶菌酶、胃蛋白酶、人α-酸性糖蛋白 (AGP)、鸡卵白蛋白和鸡卵糖蛋白（命名为鸡 AGP）。
